FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15421432
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189710.12301711021388935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG27200.09.0065455
CTTATAC10800.08.9407963
TATATCG2202.5403115E-68.9082925
TAGACAG25250.08.7596115
TATACTG18350.08.6204815
CTAGGAC22300.08.4718793
ATACCGT4153.6379788E-128.4338836
GTGTTAG23800.08.2936971
TAGGACT20550.08.1052244
CTACACT28050.07.9340354
TCTATAC12500.07.8368023
GTCTAAG15550.07.83240031
GTGTATA27800.07.80535941
TAGACTG18750.07.7646375
GTGTAGA20750.07.69117641
CTAGTAC9650.07.68598273
TACACAG34500.07.6283015
TGGACTG24550.07.58384135
CTAGAAC20650.07.52235363
TGGACCG10050.07.52171375
Produced by FastQC (version 0.11.5)