FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17946938
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237350.13225097228284846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG21400.08.76405055
CTAGGAC28450.08.4623833
TAGACCG5700.08.3486815
CTAGTAC11800.08.0662823
TACACTG31300.08.0490015
CCTATAC16600.07.8419013
GTCCTAG29350.07.7790711
GTGTATA31250.07.7095081
TAGGACT27150.07.68128544
GTCTAGG25750.07.45232151
GTCTTAG21650.07.44026141
CCTACAC28600.07.4391633
TATGCCG3208.604784E-77.43554455
TAGACAG31950.07.40337375
TAGGACC19100.07.4012574
TAGACTG23850.07.39428145
AGGACTG42250.07.3874065
TCTACAC23900.07.37936833
CTACACT31300.07.37831264
TAGGACG7600.07.36654574
Produced by FastQC (version 0.11.5)