FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589005
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT465181.7967520340825918No Hit
GAAGCAGTGGAACCTT149250.5764762910847989No Hit
GTTCTTCCTAACGTAA139670.539473658799423No Hit
TGTCCCATGAGACAAT105320.40679720587638885No Hit
CATGGAGGATCGACAT105160.40617920784239503No Hit
CGCTTAAGATAATAGG104700.404402463494663No Hit
AAAGCATGATCTTTGT97780.37767404852443315No Hit
TGAGCGCACAGGACAC91020.35156363158819703No Hit
TGTCGAAACTTCGCAA89670.3463492731763747No Hit
GATACGTACCGCTCGA89240.3446884034600165No Hit
TAATTGCCTGTCTGGC88050.34009204308218793No Hit
GGAAGGACTAGGTAAT79180.3058317770726592No Hit
GGGGGGGGGGGGGGGG74500.28775533457834185No Hit
CATGTCAGAGGTTTGT69060.26674340142255426No Hit
AATGATGGAAAGCTAA68990.26647302728268196No Hit
TCGACCTTGTTTCGCC65810.2541903163570561No Hit
GTGAGATCTCGATTAC59380.2293545203659321No Hit
GCTATGATGACCTGCG56400.2178443069827984No Hit
ATGGGTACTTGGCTGC52510.20281922978132527No Hit
AAACGACCTAGGGAGG51300.1981456196492475No Hit
CAGCTCACTGTTTACG48720.1881804013510982No Hit
AGCCTACCTTAACTCG48660.18794865208835054No Hit
TTGCGATGACATTCAG47890.1849745365497556No Hit
TGTTCCTCTTTATGGC47480.1833909165876466No Hit
CAATGTGACCAACGCC44450.17168757881888988No Hit
AGCTTGTGACTCACTC43650.1685975886489211No Hit
CTGCCTTGACCATAAC42960.16593247212732304No Hit
TAACCAGACATCACAC42570.16442610191946325No Hit
CCTGTTGACCCTAGTA42500.164155727779591No Hit
CACAGCTCTGGACTGA42280.16330598048284958No Hit
CATGATGTGTCATGTG41110.15878686985927026No Hit
CATGCAAACGAAGGCT40990.15832337133377494No Hit
ATGGGTACTTTCGGGC40450.15623762796904603No Hit
CTATTCAACCGAACGA39730.15345663681607413No Hit
ATGCCGCGATGCCACG39530.15268413927358193No Hit
AGAACGCGAGGAATAT38430.14843540278987485No Hit
TTGTTTGTGATACGCT37770.14588616089965062No Hit
ATTAGCGTGGTCGGTC37410.1444956653231647No Hit
CACTATACTACGCCAC36600.14136705027607133No Hit
GCAGATACTCGGCTCA36340.14036280347083147No Hit
GCATCTCTGAGCCTAT36300.14020830396233303No Hit
CATTAGTCTATTCTGG36110.13947443129696543No Hit
ATAGGAAACAGACTTC35370.13661619038974432No Hit
GGGTCGTACCGCTCGA35270.13622994161849822No Hit
CCGCTATTGTTCGCAA34650.13383519923677242No Hit
GGGATCAGATGAGTAA34340.1326378280459095No Hit
CTGCCATTGCACAGTT34170.13198120513479117No Hit
TTGACGTGACCTCAAA34000.1313245822236728No Hit
AACGTCCCTTACAGAA33890.1308997085753021No Hit
AGCTCGGACCTTAAGC33780.13047483492693138No Hit
TGGAGTTACAAGTCTC33640.12993408664718686No Hit
CCTACGGGATGGAACG33370.1288912149648224No Hit
CCCAATGCTGGAATGC33350.12881396521057317No Hit
AACCAACACCGGAATG33160.12808009254520558No Hit
TTGTAACCTCATCCTC32900.12707584573996572No Hit
TGGGCCATGCGTTCTC32640.1260715989347259No Hit
TCAGGCTCTTAAAGCA32640.1260715989347259No Hit
ATAGCCAACATGGCCC32450.1253377262693583No Hit
CTGATTACTGCTTTGA32430.1252604765151091No Hit
TGTTTGTTGCGCTCGA32020.12367685655300009No Hit
CGATCAAACCGATCGC31520.12174561269676962No Hit
GATACCATGTCTGGAT31250.12070274101440513No Hit
CTCGGATCTTTCTGAG31100.12012336785753601No Hit
CGCTTACTGGCCACGT30820.11904187129804693No Hit
TTCTAACACTACGTTG30560.11803762449280708No Hit
ATCTTACTGCGCACTG30530.11792174986143325No Hit
TCCAAAGTGAAGGCCA30520.11788312498430865No Hit
CGCTTCCCTGCGCTGT30250.11684025330194417No Hit
CGCTGAGTGTTAGCTG30240.11680162842481957No Hit
GCGTCGATGCTTGAAC30120.11633812989932425No Hit
CATGTTACTAACGTGG29950.1156815069882059No Hit
TGGGTGCACTTCTGAG29680.11463863530584144No Hit
CCTCCCTTGTTTCGCC29500.11394338751759847No Hit
CTTAGGCCTTATGACC28860.11147139538162344No Hit
GTAAGAAACGACAAAT28750.11104652173325273No Hit
AACCACTGAAAACATG28730.11096927197900351No Hit
CGAGTCTCTTACTTTC28580.11038989882213437No Hit
ATTGAGAGATGATCAC28450.10988777541951446No Hit
AACAGCTACTCGAGAG28420.10977190078814061No Hit
TACAACCGATTCCATT28410.109733275911016No Hit
GCATAGTACGCTCCTT28400.10969465103389141No Hit
TACGCCTTGGGTTGAG28390.10965602615676678No Hit
TATCTCCCTGGAATTA28330.10942427689401912No Hit
AGTGGTACTCAACGCC28070.10842003008877928No Hit
TGAGATAGACTCATCC28000.108149655948907No Hit
GCGACAAGAAAGGCTG27930.10787928180903475No Hit
GGTAGCCTGTGCCACG27850.10757028279203787No Hit
ATCGCAATGTAACTCG27660.10683641012667028No Hit
GCATATCGAGCCGCTA27580.1065274111096734No Hit
CCTTCAGTGTAGCTTG27410.10587078819855504No Hit
TGGTCCTTGTTTCTGT27260.10529141504168589No Hit
GAAAGTAACCCTGCGA27130.10478929163906597No Hit
TTCACGCTGTGGGCAA27110.10471204188481677No Hit
TTCTATGACCAACGCC26860.10374641995670152No Hit
GGTCAGAGAACCAGTT26680.10305117216845854No Hit
AGAACAATGAGTCGGA26670.10301254729133394No Hit
CATGTCAGAACAGGTG26540.10251042388871401No Hit
AGCCGGTACGCGCAAA26540.10251042388871401No Hit
GTTGCTAGACGCTCGA26510.10239454925734018No Hit
GGGCTGACTAATACAG26500.10235592438021555No Hit
GTGTACTGAATCACAC26500.10235592438021555No Hit
AGGTTACTGGTCCCGA26400.10196967560896945No Hit
ATTCCTAACATTGCCA26270.10146755220634954No Hit
AGAGAATTGCCCGCAA26130.10092680392660501No Hit
ACCTCCATGGAATTGA26050.10061780490960813No Hit
CCTTCTTTGGATCATC25950.10023155613836203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)