FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369301
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCAA324836096.41050176282855No Hit
TTAGTCAA700962.080431519772202No Hit
TCAGACAA87710.2603210576911947No Hit
TCAGTAAA66550.19751871382224384No Hit
TCAGGCAA66100.1961831252238966No Hit
ACAGTCAA60720.18021542153698944TruSeq Adapter, Index 13 (100% over 8bp)
TCAGTCAT36010.10687676761441023No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)