FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18034278
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG264390.14660415016337222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG5900.08.777274
TAGACTG25500.08.7269875
CTAGACT27450.08.4639974
TATACTG21650.08.4041315
TCTAGAC20000.08.188273
TACACCG7600.08.1030115
TAGGACT28300.08.0119524
TAGACAG31350.07.99145
CTAGGAC28500.07.9071013
GTCCTAA21350.07.87379031
TACACTG30650.07.76293235
GCACCGT15300.07.5931676
TGCACCG19200.07.5812265
GTCCTAG27550.07.576441
TAGACCG7050.07.5440035
CCTATAC16300.07.4708143
GTATAGG16950.07.4382931
CTAGCAC27250.07.3966023
GTGTATA35350.07.37095931
AGGACCG17300.07.36211545
Produced by FastQC (version 0.11.5)