FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20423124
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229530.11238731155919143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG25700.08.9874235
TAGACCG7800.08.6145185
TACACTG37400.08.4590435
TATACTG22400.08.3116645
CTACACT33000.08.0164314
GTCTTAG24550.07.86787751
CCTATAC16400.07.850233
TACCCTG29700.07.8241295
GTCCTAG28650.07.81673431
CTAATAC22650.07.784683
TCTACAC26100.07.7207663
TACACCG8100.07.6041735
GTATTAG19300.07.54233841
GTGTTAG28800.07.53302151
GTCCTAA22450.07.4816081
CTAGGAC29000.07.4794923
CTTACAC24900.07.4746473
AGACCGT13150.07.45287376
TTACACT28400.07.4420374
GTATAGA16000.07.4358221
Produced by FastQC (version 0.11.5)