FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20035225
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT6200.09.0306766
TAGACTG24100.08.8283285
TACACCG8350.08.7170355
TAGACCG8050.08.6941295
TATACTG24600.08.59198955
TAGACAG30350.08.3016755
CTAGGAC27900.08.2279493
TAGGACC19350.08.1742784
CTAGTAC13850.08.0852253
AGGACCG21700.07.9985985
GTATAGG15250.07.98777871
CTACACT33200.07.96848344
TATACCG4256.548362E-117.9044975
TACACTG33650.07.9035185
GTCTTAG24150.07.8269621
TCTATAC16450.07.7433243
GTGTAAG27650.07.6464251
GTCTAAG20250.07.60578871
CGTCAGA29100.07.6022241
GTGTAGG29500.07.49914361
Produced by FastQC (version 0.11.5)