FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16409080
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG6700.09.8203675
TCTATAC12750.09.0034363
TACACTG27700.08.8948285
TATACTG19400.08.4428445
TAGACTG22750.08.368785
GTATAGA16550.08.2904681
CCTATAC13300.08.2100843
GTGTAGG26450.08.0457931
TAGGACC16200.08.0366044
CTAGGAC24450.08.0159283
GTATTAG18350.08.0113221
CTTACAC19400.07.9377173
CTATACT18550.07.92410144
GTAGGAC16400.07.85323523
AGTACCG8400.07.66625365
TCTAGAC17350.07.66529273
CGTCAGA25750.07.61205241
GTATATA29950.07.5730211
CTAGACA24250.07.56247854
TACACAG36700.07.55272675
Produced by FastQC (version 0.11.5)