FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3504907
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT335461495.71192616523064No Hit
CTTTCCTA644911.8400202915512451No Hit
CTTTCCAT100180.2858278407957758No Hit
CTTTACTT98030.27969358388111293No Hit
CTTTCATT88670.2529881677316973No Hit
CTATCCTT63870.18223022750674983No Hit
CATTCCTT63510.18120309611638769TruSeq Adapter, Index 27 (100% over 8bp)
ATTTCCTT62230.17755107339510007No Hit
CGTTCCTT53260.15195838291857672No Hit
CTTACCTT49070.1400037147918618No Hit
CTGTCCTT46560.1328423264868369No Hit
CTTTCCGT46060.1314157551113339No Hit
CTTTCGTT39330.11221410439706389No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)