FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015048
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT291426496.65730031495353No Hit
CTTTCCTA538581.7863065529968345No Hit
CTTTCCAT72150.2392996728410294No Hit
CTTTCATT51040.16928420376723685No Hit
CTTTACTT49870.16540366853197694No Hit
CATTCCTT39860.13220353374141972TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT38350.1271953216001868No Hit
ATTTCCTT32300.10712930606743244No Hit
CTTACCTT30290.10046274553506279No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)