FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30060059
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC31850.011.95628122
CACGAGA38700.09.83931721
CTTATAC43200.09.789121
TCTCCGA39850.09.76605411
ATCTCCG39500.09.67538410
CCCACGA40150.09.4839919
TATACAC63350.09.3695873
TATACTG38350.08.5417885
GACTCTA46350.08.48900526
TACACCG14000.08.2994325
CCACGAG46350.08.27571720
TACACAT71850.08.16367155
ACTCTAT47900.08.15623727
GTATTAG33550.08.0553651
TAGACCG11350.08.0170735
CTAGGAC45550.07.8370573
TCCGAGC52650.07.79069513
GGACTGT52150.07.7847156
TAGGACT45600.07.7056514
TAGACAG52550.07.6988075
Produced by FastQC (version 0.11.5)