FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30060059
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG448250.14911813712674352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC45600.08.31428053
TAGACAG51700.08.2567525
TATACTG32300.08.1028735
GTGTATA52250.07.97851371
TAGACTG39650.07.9421715
TAGGACC29850.07.8281224
GTATAGG24850.07.8249291
GTGTAGG47750.07.8222181
GTACTAG18600.07.82190281
TACACTG54700.07.7783275
GTCTAGA37400.07.74266961
CGTATAC5350.07.5834093
GTTCTAG38200.07.5438991
TACACAG67750.07.4782365
GTATAGA27100.07.33011961
GTCTTAG37150.07.26759151
TAGGACT41800.07.2638844
CCTACAC49300.07.17949443
GGACTGT51950.07.16735746
GGACCGT17600.07.15801866
Produced by FastQC (version 0.11.5)