FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30810208
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGG921610.299124887439903No Hit
CTTAGAGACAATCGCT325240.10556241619660602No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTC23550.010.001636510
AGCGGTA29350.010.0013289
GTCGTAG4850.010.00132759
CGCAGGA10300.010.00132759
ATCGGCG10650.010.0010521
GTCGGCT1800.010.0010511
AGGTCGA25150.010.0010511
CTCCGGC11050.010.0005658
ACGTCGG2850.010.0005658
GTGGTCT1550.010.00057
CGAAGTC15450.010.00057
CCTTCGC5600.010.0004997
GGAGCCG7650.010.0004997
ATGTATT3900.010.0004997
GACGCGG12250.010.0004997
CACCGGG9350.010.0004997
CGTCTCG4500.010.0004355
GGTCTTA9000.010.0004355
CGGAGAT4650.010.0004356
GGGCTCA2150.010.0004345
Produced by FastQC (version 0.11.5)