FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30810208
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC33400.011.61060422
CTTATAC43300.010.7703751
ATCTCCG39650.09.921329510
CCCACGA40200.09.78581119
TCTCCGA41300.09.6944411
CACGAGA41200.09.5823521
TATACAC70250.09.4059143
TATACTG36000.09.0606085
TACACCG14100.08.5385325
CCACGAG48300.08.49254320
TACACAT78350.08.1118665
CTAGGAC47300.08.1095133
TCCGAGC53000.08.08270113
TTATACA62000.08.0383152
TACACTG56350.08.0243995
TCTATAC25000.07.95158433
TAGACTG42600.07.8868865
TAGACAG55600.07.8808575
GTAGGAC29400.07.80911543
GTATAGA29500.07.69072961
Produced by FastQC (version 0.11.5)