FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30728118
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405360.13191826456797648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG34400.09.4802915
TAGACTG39800.08.6863645
TACACTG53500.08.3195065
TAGACAG53500.08.1886955
TACACCG14200.08.0825745
TAGGACC30750.08.054944
GTCTTAG39850.08.0391021
CTAGGAC46050.07.9908773
GTATAGG25600.07.92370651
TCTAGAC34650.07.79331873
GTGTAGG50700.07.6983311
GTAGGAC26950.07.68371533
GGACCGT17400.07.6427236
CCTACAC50100.07.59625433
CCTATAC23650.07.51347643
CTATACT33950.07.50180634
GTGTATA53900.07.3710591
TATACAG40150.07.32078935
CTACACT54800.07.2777734
CTTATAC27550.07.2624433
Produced by FastQC (version 0.11.5)