FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30728118
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGG1041720.33901197593682764No Hit
CTTAGAGACAATCGCT310580.10107355094119334No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGGA11000.010.0020989
CAGTAGG8200.010.0020979
CTAAGGT14300.010.0015598
CCTTCGA4400.010.0005827
AACGTCT3500.010.0005827
ATGTCGT11300.010.0005827
AGAGTTT7650.010.0005827
ATGGTCG1650.010.0005827
GCACGTC16900.010.0001756
GTCTTAG8450.010.0001756
TAGAGTT1600.010.00017456
CGACGTC2650.010.00017456
AGTGTAT5350.010.00017456
GGCTCCA5600.010.0001746
GGGCTAA1600.09.9997515
GCAGATC2000.09.9997515
TCCGCTT6850.09.9994584
TAGTACA12000.09.9994584
CGGCACC652.2080876E-69.9994574
CAAATGT17150.09.9994574
Produced by FastQC (version 0.11.5)