FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3056930
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT335681.0980951477462684No Hit
TGCTGCTACCGTTCTC305931.0007752876251665No Hit
AAACGACCTCTTCAAG303340.9923027350969764No Hit
TATGCTTTGGACTGTT293620.9605061286977458No Hit
ATGGGTTACAATCGAA291690.9541926049991332No Hit
GCCTCTTGATCTGTGA272260.8906321047586959No Hit
AAAGCGGACTCGGGCT267470.8749627894652479No Hit
TCCAAAGTGCTCACGG259130.8476805160733155No Hit
TAGTGCATGAACCCGC239060.7820264121193484No Hit
GCAGGTCTGGCTGTTA229820.7518000085052651No Hit
TAACTCACTGGTGTCC228760.7483324773547317No Hit
TGTGGCTCTTACGATC227250.7433928810931228No Hit
GAAGTATGAACAGGTG216380.7078343305211438No Hit
TTCCCATCTCTTTGTT208830.6831363492130994No Hit
GAGCCTGACCGAGTTT195490.6394977968092171No Hit
TCACCTGACTCAATGC193580.6332496982266522No Hit
GGAACTACTACCAAGG191460.6263146359255855No Hit
TGAGAATGAGCAGTTT189460.6197721243208055No Hit
ATGTCCAGAGTCGGTC181090.5923917132548014No Hit
CTAGAGACTAATCTGC179080.5858164890919975No Hit
TCGTATACTGCTAATC175040.572600615650342No Hit
TCACACAGATTGGGAG173680.5681517077590916No Hit
TTCGTCCGACCTGTGG173570.5677918696208287No Hit
ATTCCTAACTGCTACA172110.5630158361493394No Hit
AGGTCGAGAAGGGAGG168890.5524823924656437No Hit
TCCGCGAGAGAACAGC168270.5504542138681618No Hit
ACTGATTCTCGGCTCA165010.5397899199523705No Hit
TGGACCTCTAGGGAGG163080.5334763962537579No Hit
TGGCCTGGACCCGAGT160200.5240551795428747No Hit
CCATGATGATTCGGGC157820.5162695907331866No Hit
GCAATCATGGAACGTG156670.5125076465604381No Hit
TTCGATGGAAATCCTG140320.4590226141913619No Hit
ACGAGGATGGAGCTGA137030.4482601826014989No Hit
AAGGTGCGAACAGATA134340.43946050449306984No Hit
TCCCAGACTGTCTTGA132880.4346844710215804No Hit
TTTCGTAACAGTACGG132760.4342919203252937No Hit
TGCGGTGACCTTAGTG129110.4223518366465702No Hit
TGGTCCTTGGAGAGGG129080.4222536989724986No Hit
TCTCTCTACTCTGGAT127760.41793564131334376No Hit
TCCTTCACTTGTCACA112910.36935749264785256No Hit
TTCGATGGACAGGTGA112470.36791814009480095No Hit
TGATATCCTCGATGTT111960.3662497996355821No Hit
TCGCCGAGAAGGTAAT109030.35666502013457946No Hit
AGCCCAGTGAAAGTAC108340.35440785363093036No Hit
AGCTGCTCTTGTCACA107730.3524123875914725No Hit
CTTTCGGTGCTTTGTT106200.34740736621381585No Hit
AACTCCCTGCTTCACC105990.34672040249531394No Hit
AACCCTCACGCGGTTC104250.3410284173991554No Hit
CCCTTGCCTCCTACGG100570.32899019604636026No Hit
ATGGCCGTGATACGCT100180.32771440628342813No Hit
GAAGTAATGAGTCTGT98020.3206484937502658No Hit
TCGGCTTACCTCCTAC95520.3124703542442908No Hit
AAACACGCTGCAGTTT93690.30648395612591717No Hit
TGTTTGTTGCAAGTTC92870.30380152636795743No Hit
GGGGGGGGGGGGGGGG89930.29418403430893086No Hit
CTAACTGGAACCTTGA89270.2920250054793535No Hit
TGGCCTTTGCGAAAGC88970.2910436287386365No Hit
ACCCTTGTGGGCCATC88790.29045480269420626No Hit
TACAACGTGAGCAGGT87320.285646056664693No Hit
CAATGTGTGGTTGACT86580.28322532737092443No Hit
TCAAAGGCTCTTAGTG84000.2747854874007583No Hit
AAATAGGACTCTATCT83730.273902248334113No Hit
GGACATTTGGCCGCTA81880.2678504250996915No Hit
ACAGCCGACAAACATG81860.2677849999836437No Hit
CTTTCGGTGAGCCATT80090.2619948772134134No Hit
TGAACTGTGTGCTGGC79790.26101350047269645No Hit
AGTGTTCGATTCGCCT75780.24789576470511265No Hit
CAGCCGATGTAATAGG75610.24733965121870638No Hit
GGCTTGCGAATACGCT70450.23045997127837406No Hit
GATAAGAACATTGGTC66590.21783292388114872No Hit
GTAAAGCCTGAAATCA64640.21145397506648825No Hit
TTGGGAGGAATCTGTG63930.2091313834467914No Hit
GTGCTAATGGGAGTCA62960.2059582653184731No Hit
CGACGTTCTTGCTACA62380.2040609369530869No Hit
TGTACTGGAAATGCTC60900.19921947836554973No Hit
AGGGACCCTAATCCTG59920.1960136476792076No Hit
GGACCACTGGCTAGAG59710.19532668396070565No Hit
GGAACATCTTTACGAC59070.1932330802471761No Hit
TCTTGAACTCGTACAT57410.1878027956152087No Hit
GGGTGAAACCGACTAT57380.18770465794113703No Hit
TACGTGTGAAACGCAG55780.18247064865731305No Hit
TAACGAGCTTACTTTC55680.18214352307707404No Hit
TACCACCTGAAACATG55400.18122757145240487No Hit
AGCTTGTGAACCGTGC53960.1765169630969633No Hit
ACTTATCTGTCAGGTT53560.1752084607760073No Hit
CGGATGAGAACAGGCA53140.1738345333390035No Hit
CATGATGTGCCTAGTA51810.16948376312182484No Hit
AACTAGCACTGCCACG49850.16307210174914047No Hit
TCGGACCGAATGTCCA48030.15711841618879072No Hit
ACGTGTTGATCTTTGT47630.15580991386783474No Hit
CGTCGTACTTGCGCCA46910.15345460969011393No Hit
TGGTAGGGATGTGCTA46870.15332375945801835No Hit
AACTACGACATTGCCA46090.15077217993215417No Hit
GTAGATAGAGCTGAGA45970.15037962923586734No Hit
TTTAGGGACTGGGCAA45310.14822060040628998No Hit
AACGAACCTGACACCC44420.1453091827421629No Hit
CTCGGATCTAGTACGG44140.1443932311174937No Hit
GGTAGTTTGTCCAGCG43880.1435427046088723No Hit
CCTTCAGTGCAACGCC43740.14308472879653772No Hit
GCCATAATGACCGTTA43390.14193978926570122No Hit
AGTTTGCGAGCAGGAT42800.14000974834229113No Hit
TGAGGAGTGGTCCCAG42470.13893023392750242No Hit
GTCCGAGTGGTCTTGA42380.13863582090528734No Hit
TACCACCTGTACTTTC41820.13680391765594893No Hit
CTTTAACTGTTCGCCT41080.13438318836218036No Hit
AGAACGCGACACAGTT40200.13150448325607716No Hit
CAGGCCATGTCGTAGT39850.1303595437252407No Hit
GTCCGTTACGGATGGT38890.12721913815494632No Hit
CTTACATTGGAAGGCT38690.1265648869944683No Hit
TGCAATGACTCCGTAA38050.12447128328093872No Hit
GAGAAGAGATGCTACA37890.12394788235255633No Hit
GAAAGTCCTTATCTGG37420.12241039212543302No Hit
GGGATGTGATGCATGT37350.12218140421926572No Hit
CCGTTACTGTTGGTTG37170.12159257817483554No Hit
GAACCATACTTTGGGT36990.12100375213040535No Hit
CAAAGACCTGCTTTGA36780.12031678841190345No Hit
CGATCGGTGCGAGGAT36730.12015322562178395No Hit
AACTACGACGCTGTTA36270.11864844795268456No Hit
AATTCTGCTTAGCGAG35620.11652213168113106No Hit
GCTGGACACATTGGTC34870.11406868982933857No Hit
TCCTCATCTTTCTGGA34240.1120077986738329No Hit
GGCTTAAACTGCATCA34020.11128812239730711No Hit
GAGGTTGGAGGAATAT33800.1105684461207813No Hit
TGTCTTCCTGAAATCA33790.11053573356275741No Hit
TCGTAGAACCGCACTG33310.10896553077761023No Hit
TCCGTAAGATTACGCA32840.10742804055048692No Hit
CCTGAGGACTGACTCA32750.10713362752827182No Hit
CCCACCTCTTGACCTA32500.10631581357767433No Hit
TATGGGTACCCTTTGC32200.10533443683695734No Hit
AGGTGATCTGTTAGTC32020.10474561079252713No Hit
ATAGCCAACATCTGTG31920.10441848521228815No Hit
ACAAGACCTAAGCTAA31880.10428763498019254No Hit
ATGGCCGTGGGCCAAA31710.10373152149378624No Hit
ATCTCTTGACGGTACC30910.10111451685187428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)