FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590980
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA253662597.90214513427352No Hit
GGCAAGAA84230.3250893484318675No Hit
GGCAAGGC71430.2756871917189635No Hit
GGCAAGCA39120.15098534145381284Clontech Universal Primer Mix Long (100% over 8bp)
GGCAGGGA38970.1504064099298335No Hit
GGCAACGA37440.1445013083852442No Hit
GGAAAGGA28880.11146361608348965No Hit
GCCAAGGA27120.10467081953546534RNA PCR Primer, Index 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)