FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004947123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18024581
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1907121.0580662041464377No Hit
AAACGACCTCTTCAAG1762010.9775594783590253No Hit
TGCTGCTACCGTTCTC1754720.9735150015415059No Hit
ATGGGTTACAATCGAA1708540.947894433718043No Hit
TATGCTTTGGACTGTT1674460.9289869206945781No Hit
GCCTCTTGATCTGTGA1588210.8811356003226927No Hit
AAAGCGGACTCGGGCT1539760.8542556412268335No Hit
TCCAAAGTGCTCACGG1492690.8281413032569246No Hit
TAGTGCATGAACCCGC1404800.7793801143005765No Hit
GCAGGTCTGGCTGTTA1372030.7611993865488468No Hit
TAACTCACTGGTGTCC1338550.7426247522757949No Hit
GAAGTATGAACAGGTG1321760.7333096952434013No Hit
TGTGGCTCTTACGATC1317590.7309961879280301No Hit
TTCCCATCTCTTTGTT1184220.6570027896903678No Hit
TCACCTGACTCAATGC1125760.6245693034417832No Hit
GGAACTACTACCAAGG1116030.6191711197059172No Hit
GAGCCTGACCGAGTTT1113670.6178617966209589No Hit
TGAGAATGAGCAGTTT1095830.6079642017753423No Hit
CTAGAGACTAATCTGC1058660.5873423631872497No Hit
ATGTCCAGAGTCGGTC1046190.5804240331578304No Hit
GAAGTAATGAGTCTGT1032460.5728066577525436No Hit
TCGTATACTGCTAATC1027250.5699161606031231No Hit
TTCGTCCGACCTGTGG1013260.5621545377393239No Hit
TCACACAGATTGGGAG1009430.5600296617158534No Hit
TGCGGTGACCTTAGTG1006070.5581655407135401No Hit
ATTCCTAACTGCTACA991990.5503539860371789No Hit
TCCGCGAGAGAACAGC966900.5364341062907371No Hit
AGGTCGAGAAGGGAGG960670.5329777152656142No Hit
ACTGATTCTCGGCTCA956630.5307363316794992No Hit
TGGACCTCTAGGGAGG947370.5255989029647902No Hit
TGGCCTGGACCCGAGT924480.5128995786365298No Hit
CCATGATGATTCGGGC900830.4997786078910794No Hit
GCAATCATGGAACGTG821010.4554946381277878No Hit
TTCGATGGAAATCCTG819280.4545348377307633No Hit
ACGAGGATGGAGCTGA806280.44732246480514587No Hit
AAGGTGCGAACAGATA787560.43693664779225655No Hit
TTTCGTAACAGTACGG775380.4301792091588703No Hit
TCCCAGACTGTCTTGA770600.4275272751139125No Hit
TGGTCCTTGGAGAGGG746820.414334180639206No Hit
TCTCTCTACTCTGGAT726350.40297746727094513No Hit
GCGGTTCGAGTACGTT701250.3890520395453298No Hit
TCCTTCACTTGTCACA658170.365151345265668No Hit
TCGCCGAGAAGGTAAT647930.3594702145919509No Hit
TTCGATGGACAGGTGA639380.3547266923985639No Hit
AGCCCAGTGAAAGTAC637360.35360600060550645No Hit
TGATATCCTCGATGTT632130.3507044074977388No Hit
AGCTGCTCTTGTCACA608990.3378663836901396No Hit
AACTCCCTGCTTCACC606740.3366180883760904No Hit
CTTTCGGTGCTTTGTT606580.3365293207093136No Hit
AACCCTCACGCGGTTC601830.3338940306018764No Hit
CCCTTGCCTCCTACGG586620.32545555427890394No Hit
ATGGCCGTGATACGCT582090.32294231971328485No Hit
TCGGCTTACCTCCTAC564950.31343308340981685No Hit
TGTTTGTTGCAAGTTC540240.29972402687197003No Hit
GGGGGGGGGGGGGGGG528990.29348255030172404No Hit
AAACACGCTGCAGTTT527470.2926392574673442No Hit
ACCCTTGTGGGCCATC524730.2911191111737909No Hit
CTAACTGGAACCTTGA519170.28803443475329604No Hit
TGGCCTTTGCGAAAGC512230.284184137206851No Hit
TACAACGTGAGCAGGT501280.2781091000118116No Hit
AAATAGGACTCTATCT499150.2769273804478451No Hit
CAATGTGTGGTTGACT491470.2726665324425572No Hit
TCAAAGGCTCTTAGTG490840.2723170097546234No Hit
CTTTCGGTGAGCCATT466870.2590185036756194No Hit
TGAACTGTGTGCTGGC463980.2574151376944629No Hit
AGTGTTCGATTCGCCT435040.2413592859662036No Hit
CAGCCGATGTAATAGG427220.23702076625248597No Hit
GAGGTTGGAGGAATAT414400.22990825695199238No Hit
GGCTTGCGAATACGCT387230.21483439753745176No Hit
ACAGCCGACAAACATG381830.2118384887837337No Hit
GATAAGAACATTGGTC380840.21128923884555206No Hit
GTGCTAATGGGAGTCA377870.20964148903100718No Hit
CGACGTTCTTGCTACA376950.20913107494704036No Hit
TTGGGAGGAATCTGTG366200.20316699733547205No Hit
GTAAAGCCTGAAATCA364170.202040757563241No Hit
TGTACTGGAAATGCTC363180.20149150762505935No Hit
AGGGACCCTAATCCTG342590.19006821850671593No Hit
TCTTGAACTCGTACAT335230.18598490583498167No Hit
GGGTGAAACCGACTAT327900.1819182371007681No Hit
TAACGAGCTTACTTTC326970.1814022750376278No Hit
GGAACATCTTTACGAC326370.18106939628721466No Hit
CGGATGAGAACAGGCA321770.17851732586738078No Hit
TACGTGTGAAACGCAG320580.1778571163457281No Hit
TACCACCTGAAACATG319840.17744656588688526No Hit
GGACCACTGGCTAGAG318500.17670313667762927No Hit
GGACATTTGGCCGCTA311750.17295825073548174No Hit
AGCTTGTGAACCGTGC309280.1715878998796144No Hit
CATGATGTGCCTAGTA304130.16873069060523516No Hit
ACTTATCTGTCAGGTT300330.1666224585192854No Hit
AACTAGCACTGCCACG299240.16601772878936824No Hit
TCGGACCGAATGTCCA284310.15773459588325522No Hit
ACGTGTTGATCTTTGT283580.15732959340358593No Hit
AACTACGACATTGCCA275580.15289121006474435No Hit
TGGTAGGGATGTGCTA275170.15266374291862875No Hit
CGTCGTACTTGCGCCA269740.14965119022739No Hit
GTAGATAGAGCTGAGA266510.14785919295433275No Hit
CTCGGATCTAGTACGG266230.1477038495374733No Hit
GTCCGAGTGGTCTTGA261750.14521835486772203No Hit
AACGAACCTGACACCC261710.14519616295102783No Hit
TTTAGGGACTGGGCAA261290.14496314782573863No Hit
GGTAGTTTGTCCAGCG257680.1429603273440864No Hit
TACCACCTGTACTTTC255140.1415511406340042No Hit
CCTTCAGTGCAACGCC252890.14030284531995502No Hit
TGAGGAGTGGTCCCAG251650.13961489590243456No Hit
AGTTTGCGAGCAGGAT249790.13858297177615392No Hit
GCCATAATGACCGTTA237810.1319364927262387No Hit
CTTTAACTGTTCGCCT235570.13069374539136305No Hit
CAGGCCATGTCGTAGT232060.12874640470144633No Hit
AGAACGCGACACAGTT227760.12636077365681897No Hit
GTCCGTTACGGATGGT224800.1247185718214476No Hit
CTTACATTGGAAGGCT224260.12441898094607581No Hit
GAGAAGAGATGCTACA220600.1223884205685558No Hit
TGCAATGACTCCGTAA218790.12138423633814291No Hit
GGGATGTGATGCATGT217800.12083498639996126No Hit
CCGTTACTGTTGGTTG217300.12055758744128366No Hit
GAAAGTCCTTATCTGG212040.11763935039599534No Hit
AACTACGACGCTGTTA211640.11741743122905327No Hit
GAACCATACTTTGGGT210430.11674612574905348No Hit
TCCTCATCTTTCTGGA206290.11444926237120297No Hit
CGATCGGTGCGAGGAT206270.11443816641285587No Hit
AATTCTGCTTAGCGAG201400.11173630055533607No Hit
GAAGGACACCCAGCTG199150.1104880052412869No Hit
GCTGGACACATTGGTC198130.10992211136558458No Hit
GGCTTAAACTGCATCA196850.10921197003136993No Hit
TGTCTTCCTGAAATCA196130.10881251553087419No Hit
CAAAGACCTGCTTTGA195500.10846299284294042No Hit
CCCACCTCTTGACCTA194810.10808018227996534No Hit
ATGGCCGTGGGCCAAA192940.10704271017451114No Hit
CCTGAGGACTGACTCA192720.106920654632693No Hit
TCCGTAAGATTACGCA191930.10648236427798238No Hit
GCGTTTAGAAGTCTAC191100.10602188200657756No Hit
ACAAGACCTAAGCTAA191010.1059719501940156No Hit
TCGTAGAACCGCACTG190840.10587763454806523No Hit
ATAGCCAACATCTGTG189870.1053394805682307No Hit
TATGGGTACCCTTTGC185980.10318131666971898No Hit
AGGTGATCTGTTAGTC182400.10119514012558739No Hit
GCGTTAATGGTACGTT182360.10117294820889318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)