FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17458929
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354590.20309951429437625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2950.011.86222655
TATACTG30100.09.9051565
TACACTG37050.09.6338585
GTATAGG15750.09.1575771
TAGACAG48250.09.1382175
GTCGTAC1554.564254E-49.0342511
ATACTGT40900.08.7949456
CTAGGAC22600.08.6699033
CTACACT27650.08.6055454
GTATTAG25400.08.3798021
GTGTATA54700.08.2431351
TAATACT31800.08.2307434
TCTATAC23550.08.1418723
CTTACAC28400.08.1313063
CTAGTAC12300.08.0788193
GTCTTAC18050.08.0682631
GTATAGA21700.08.0017661
AGGACTG37650.07.99321845
TAGGACT25600.07.9824734
GTGCTAG23800.07.94292931
Produced by FastQC (version 0.11.5)