FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143463
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT335151.0661808330494107No Hit
TGCTGCTACCGTTCTC316991.0084101514794352No Hit
AAACGACCTCTTCAAG304470.9685814657274477No Hit
ATGGGTTACAATCGAA295010.9384872670681983No Hit
TATGCTTTGGACTGTT289700.9215950688778585No Hit
GCCTCTTGATCTGTGA276720.880303028857028No Hit
AAAGCGGACTCGGGCT273250.8692642477420603No Hit
TCCAAAGTGCTCACGG254260.8088531660783028No Hit
TAGTGCATGAACCCGC247140.786202986960559No Hit
TAACTCACTGGTGTCC234540.7461198048139901No Hit
TGTGGCTCTTACGATC233220.7419206143033972No Hit
GCAGGTCTGGCTGTTA221420.7043823961026422No Hit
GAAGTATGAACAGGTG209530.6665578694579831No Hit
TTCCCATCTCTTTGTT205700.654373854567399No Hit
TCACCTGACTCAATGC198710.6321372320908502No Hit
GAGCCTGACCGAGTTT194190.6177581857970016No Hit
GGAACTACTACCAAGG191750.6099960457622692No Hit
TGAGAATGAGCAGTTT191460.6090734963319117No Hit
ATGTCCAGAGTCGGTC189170.6017885370370193No Hit
CTAGAGACTAATCTGC186420.5930402234732841No Hit
TCGTATACTGCTAATC178650.5683222611495665No Hit
TTCGTCCGACCTGTGG178450.5676860201631131No Hit
ATTCCTAACTGCTACA172990.5503166412329332No Hit
TCACACAGATTGGGAG172360.5483124821256048No Hit
ACTGATTCTCGGCTCA168830.5370828287147009No Hit
TCCGCGAGAGAACAGC168280.5353331660019539No Hit
AGGTCGAGAAGGGAGG165220.5255986789092157No Hit
TGGCCTGGACCCGAGT164790.5242307607883407No Hit
TGGACCTCTAGGGAGG160870.5117604374538527No Hit
CCATGATGATTCGGGC158100.502948499791472No Hit
GCAATCATGGAACGTG151030.4804573809203416No Hit
TTCGATGGAAATCCTG139690.4443825169884296No Hit
ACGAGGATGGAGCTGA138710.44126493615480766No Hit
TCCCAGACTGTCTTGA136290.43356642021872055No Hit
AAGGTGCGAACAGATA133620.4250726030495667No Hit
TTTCGTAACAGTACGG131210.4174058991628023No Hit
TCTCTCTACTCTGGAT128500.4087848337963577No Hit
TGGTCCTTGGAGAGGG128170.4077350361687095No Hit
TGCGGTGACCTTAGTG117710.3744596325771927No Hit
TCCTTCACTTGTCACA115280.366729304591783No Hit
AGCCCAGTGAAAGTAC112980.35941253324756806No Hit
TTCGATGGACAGGTGA112350.3574083741402396No Hit
AGCTGCTCTTGTCACA110870.3527001908404839No Hit
TGATATCCTCGATGTT110050.35009160279602464No Hit
TCGCCGAGAAGGTAAT110010.34996435459873393No Hit
AACTCCCTGCTTCACC109630.34875549672447237No Hit
CTTTCGGTGCTTTGTT106020.33727134691898714No Hit
CCCTTGCCTCCTACGG104190.3314497418929378No Hit
AACCCTCACGCGGTTC103400.3289365899964466No Hit
ATGGCCGTGATACGCT102200.32511914407772574No Hit
TCGGCTTACCTCCTAC95280.30310520594643553No Hit
TGTTTGTTGCAAGTTC94570.30084655044452563No Hit
GGGGGGGGGGGGGGGG94180.2996058805209414No Hit
AAACACGCTGCAGTTT93060.2960429309968019No Hit
CTAACTGGAACCTTGA91220.29018951392142994No Hit
TGGCCTTTGCGAAAGC91170.2900304536748166No Hit
ACCCTTGTGGGCCATC90980.28942602473768575No Hit
TACAACGTGAGCAGGT88610.2818865690482121No Hit
AAATAGGACTCTATCT88130.28035959068072375No Hit
CAATGTGTGGTTGACT85770.2728519470405728No Hit
ACAGCCGACAAACATG85160.2709114120318897No Hit
TCAAAGGCTCTTAGTG85130.27081597588392164No Hit
GAAGTAATGAGTCTGT83780.2665213492253607No Hit
CTTTCGGTGAGCCATT83410.26534430340042175No Hit
GGACATTTGGCCGCTA80720.25678686213262253No Hit
CAGCCGATGTAATAGG78200.24877022570330876No Hit
TGAACTGTGTGCTGGC78110.24848391725940466No Hit
AGTGTTCGATTCGCCT77450.2463843220041082No Hit
GGCTTGCGAATACGCT69000.21950314032644888No Hit
GTAAAGCCTGAAATCA67020.21320435456055947No Hit
GTGCTAATGGGAGTCA65190.2073827495345102No Hit
TTGGGAGGAATCTGTG64750.20598301936431254No Hit
TGTACTGGAAATGCTC63950.20343805541849863No Hit
GATAAGAACATTGGTC63190.20102033966997546No Hit
CGACGTTCTTGCTACA62630.19923886490790568No Hit
TAACGAGCTTACTTTC60710.19313095143795236No Hit
GGAACATCTTTACGAC60380.19208115381030413No Hit
GGACCACTGGCTAGAG60120.1912540405279146No Hit
AGGGACCCTAATCCTG59360.18883632477939138No Hit
TCTTGAACTCGTACAT59270.18855001633548732No Hit
GGGTGAAACCGACTAT56870.18091512449804562No Hit
CGGATGAGAACAGGCA56520.17980170277175203No Hit
TACGTGTGAAACGCAG55240.1757297604584498No Hit
TACCACCTGAAACATG55230.17569794840912714No Hit
AGCTTGTGAACCGTGC54350.17289848806873184No Hit
CATGATGTGCCTAGTA54160.17229405913160104No Hit
AACTAGCACTGCCACG53760.1710215771586941No Hit
ACTTATCTGTCAGGTT52080.1656771528724849No Hit
ACGTGTTGATCTTTGT49430.15724695980197637No Hit
TCGGACCGAATGTCCA48990.15584722963177872No Hit
AACTACGACATTGCCA48770.1551473645466799No Hit
TGGTAGGGATGTGCTA48560.15447931151090374No Hit
CTCGGATCTAGTACGG47550.15126629452931367No Hit
GTAGATAGAGCTGAGA46770.14878495468214514No Hit
GTCCGAGTGGTCTTGA46080.14658992327888065No Hit
CGTCGTACTTGCGCCA45760.1455719377005551No Hit
TTTAGGGACTGGGCAA45670.14528562925665103No Hit
AACGAACCTGACACCC45580.14499932081274697No Hit
GGTAGTTTGTCCAGCG44860.14270885326151445No Hit
CCTTCAGTGCAACGCC44450.14140455923928483No Hit
TACCACCTGTACTTTC44350.14108643874605808No Hit
CTTTAACTGTTCGCCT44230.140704694154186No Hit
GCCATAATGACCGTTA43820.13940040013195637No Hit
AGTTTGCGAGCAGGAT43610.13873234709618024No Hit
TGAGGAGTGGTCCCAG43580.1386369109482122No Hit
CAAAGACCTGCTTTGA40890.13007946968041298No Hit
CAGGCCATGTCGTAGT40270.1281071226224072No Hit
GTCCGTTACGGATGGT39740.12642108400830548No Hit
GGGATGTGATGCATGT38740.12323987907603812No Hit
CTTACATTGGAAGGCT38740.12323987907603812No Hit
AGAACGCGACACAGTT38660.12298538268145673No Hit
CGATCGGTGCGAGGAT38170.12142659226464571No Hit
TGCAATGACTCCGTAA37870.1204722307849655No Hit
GAACCATACTTTGGGT37460.11916793676273589No Hit
CCGTTACTGTTGGTTG37390.11894525241747717No Hit
GAAAGTCCTTATCTGG37070.11792726683915161No Hit
GAGAAGAGATGCTACA36550.11627304027437256No Hit
AATTCTGCTTAGCGAG36420.11585948363317781No Hit
CCCACCTCTTGACCTA36130.11493693420282025No Hit
AACTACGACGCTGTTA36090.11480968600552958No Hit
TCCTCATCTTTCTGGA35930.1143006932163668No Hit
GCTGGACACATTGGTC35310.11232834615836101No Hit
TGTCTTCCTGAAATCA34470.10965613401525641No Hit
CCTGAGGACTGACTCA34300.10911532917677097No Hit
TCGTAGAACCGCACTG33890.10781103515454134No Hit
TCCGTAAGATTACGCA33550.10672942547757043No Hit
ATAGCCAACATCTGTG33500.10657036523095706No Hit
GGCTTAAACTGCATCA33450.10641130498434372No Hit
ACAAGACCTAAGCTAA32980.10491613866617804No Hit
ATGGCCGTGGGCCAAA32730.1041208374331112No Hit
TATGGGTACCCTTTGC32370.10297560365749495No Hit
AGGTGATCTGTTAGTC31570.10043063971168104No Hit
ACTGTCCACGTTGGTT31500.10020795536642231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)