FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896845
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA283741797.94852675928468No Hit
GTGGCGCA135320.4671288936757058No Hit
GGGGAGCA49590.1711862388218907No Hit
GAGGAGCA41040.14167137005949576No Hit
GTGGAGAA35220.12158054711246201No Hit
GTGGAGCT34150.11788687347786989No Hit
GTGGAGCC33120.11433128110064569No Hit
GTGCAGCA32600.11253622475486261No Hit
GTGGACCA31540.10887707143461248ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)