FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17639304
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG20100.09.6798415
ATACCGT4150.08.7694986
TAGACCG5550.08.5750265
CTAGGAC25750.08.4800433
TAGACAG29550.08.3369015
CCTACAC24900.08.3198033
TAGGACC18350.08.08575254
GTCTAAG18050.08.0673321
TACACTG31400.08.0240265
GTCTAGG24500.07.94369461
AATACCG6250.07.83858165
AGGACCG19750.07.79605775
TACACCG7900.07.7960575
TCTATAC14600.07.76574043
CTAGTAC10700.07.7182513
CTTACAC22050.07.68117143
GTACCGT6400.07.65486436
TGGACTG26700.07.54919625
GAACCGT7500.07.4653166
CTATACT19300.07.39764644
Produced by FastQC (version 0.11.5)