FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17983643
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206010.11455409785436689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG20200.08.8702075
TAGACTG22700.08.5716435
TACACTG30400.08.5647465
TCTACAC23750.08.3077893
GTCTTAG22000.08.2074281
CTACACT28100.08.0190354
CTAGGAC26400.08.0039253
TCTATAC15400.07.9963523
TAGACCG7400.07.94497785
CTTATAC15400.07.9054843
TACACCG8700.07.88409665
GTGTTAG27050.07.86531931
GTCCTAG25150.07.84731631
GTGTATA30700.07.79646641
TAGTACG3601.1448719E-87.7755314
GTCTAGG23300.07.6293561
CTTACAC22850.07.59391073
GTGTAGG27050.07.55484631
GCTATAC10750.07.55004453
TAGGACA22450.07.5434774
Produced by FastQC (version 0.11.5)