FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773670
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG361887095.89789250252407No Hit
GGCCAGGG504461.3367888554113103ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA430911.1418857504763267No Hit
GGACAGGC69910.1852573224473788No Hit
GGACACGG61030.16172585308201298No Hit
GGAAAGGG51780.13721390582642362No Hit
CGACAGGG46890.12425569803400933No Hit
GGACAGCG43110.11423892391226578No Hit
GGTCAGGG37780.10011474241255859No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)