FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037818
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT390539796.7204812103963No Hit
CTAGCTGA237600.588436625920237No Hit
CTAGCAGT90940.22522065135179445No Hit
CTAGCTGG87500.21670119851860586No Hit
CAAGCTGT80850.2002319074311918No Hit
CTCGCTGT78500.19441193238526353No Hit
CTAGCGGT77070.19087041565518803No Hit
CTAGATGT76210.18874055244689086No Hit
CTAGCTCT70440.17445065627029252No Hit
CTAGCTAT69860.1730142368972549RNA PCR Primer, Index 38 (100% over 8bp)
CTACCTGT64950.1608542039289537No Hit
ATAGCTGT62340.15439031675028445No Hit
CTAGGTGT49580.12278909054345688No Hit
CTTGCTGT48550.12023820786375215No Hit
CTAGCTTT43630.1080534090441917No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)