FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856003
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC45960.5369140061425018No Hit
CTCCTAGTGAACGTAA44360.5182224828651302No Hit
CGACCAGACGCGCTGT43120.503736552325167No Hit
TAGCTTTACCCTATCC37850.4421713475303241No Hit
CCAGGACTGTAACTCG29710.3470782228566956No Hit
GTTCTCGACCGTAGAG27990.326984835333521No Hit
CTTCCTTTGGCTCAGC27130.3169381415719338No Hit
ATCTGTACTGGGACTA26900.31425123510081154No Hit
CGATCAAACCCGGCTA25390.29661111000779206No Hit
AGACCATTGAATCCAC23620.2759336123821996No Hit
TGCTGCTACTTTCTTG22130.2585271313301472No Hit
GGCAACAGAATAGCAA22080.2579430212277294No Hit
GGGGGGGGGGGGGGGG21300.24883090363001065No Hit
ACAGCCGACTTGCGTA20240.23644776945875187No Hit
AACTTACCTCACCTAA18100.21144785707526725No Hit
CGCGTTGGAGCGGTTC17820.20817684050172722No Hit
ATCGAAAGATTTCCGC17150.20034976512932784No Hit
GTAACTGTGTGAAAGT16660.19462548612563274No Hit
CCCTGAACTAGCTTTG16030.18726569883516764No Hit
CAATTCGGATAACGGG15900.18574701256888118No Hit
AGTGGAGTGAGTTGAA15770.18422832630259472No Hit
AAGAACCCTTAACCCA14010.16366765069748587No Hit
GGGTAGAACCAGTCTA13890.162265786451683No Hit
CGCGATCGATATCTGG13480.1574760836118565No Hit
TTCCTAAACTAATGCC13340.15584057532508647No Hit
GTGAAGCGATCCCAGG13030.1522190926900957No Hit
TGGAACACTCCTAGTA13010.15198544864912855No Hit
TTTAGTCTGAGCAATA12900.15070040642380927No Hit
TCTCAGAGATGCATGT12710.1484807880346214No Hit
TGCAGATACTGCCACG12370.1445088393381799No Hit
CACATCCTGAGCCTAT12290.1435742631743113No Hit
CTTTAACTGTCCGTAA12220.1427565090309263No Hit
TCCGGGAACGTTCTCC12010.14030324660077126No Hit
TGATAGAGAGACTGTT11940.13948549245738623No Hit
GCAACTTGAAGGTGCA11770.1374995181091655No Hit
AGTCCTGCTCGAACGA11710.13679858598626407No Hit
CATAGGTACAAGGTGC11610.13563036578142834No Hit
CTATTGGGACTTGTGA11520.1345789675970762No Hit
ATTTCTGACGGGTAGT11440.13364439143320758No Hit
TGCAGTATGACACCGC11290.131892061125954No Hit
CTTAGATCTTGTCACA11260.13154159506450327No Hit
TCAAAGCACTCTGCAA11050.12908833263434824No Hit
TGGCAATTGAGTGGTG11040.1289715106138647No Hit
AGGTGTGGATCATGTG11010.12862104455241397No Hit
ATGTACGTGTACTTTC10970.12815375647047966No Hit
CGAGTCTCTGCTACGC10890.12721918030661108No Hit
TGAACTGTGAACGTAA10850.12675189222467678No Hit
GAGTGTTACACCTGCG10830.12651824818370963No Hit
TCTATGGGATGCTCAA10690.1248827398969396No Hit
AGAGTACCTTAATCGC10650.12441545181500532No Hit
TTCTTAGACGTCCCGA10490.12254629948726815No Hit
TACGTTTACTGCACCG10290.1202098590775967No Hit
GGTCTAAACTCCTACC10240.11962574897517882No Hit
TCCATTTGAAATCTGC10200.11915846089324454No Hit
TACTGTTTGCTTAAGC10170.11880799483179381No Hit
GCATGATACTTGCGTA10050.11740613058599093No Hit
CTACAACGAATCTGTG10030.11717248654502378No Hit
GGTCGCATGGTACTAC9970.11647155442212236No Hit
GGGTGTGCTCGCAAAG9860.11518651219680305No Hit
CTCAATTTGGAATCTC9820.11471922411486876No Hit
GGGATGTGACTGGAAG9750.11390146997148376No Hit
CATTCTATGCCTAGCG9730.1136678259305166No Hit
CACCAGGTGCGCCACA9700.11331735986906588No Hit
AGACTACTGGCCCTAG9630.11249960572568088No Hit
GTAATCTCTACACAAA9620.11238278370519729No Hit
TTGCGGTCTTTTGGGT9570.11179867360277944No Hit
CTAGCTCACGAATAGT9440.11027998733649298No Hit
CTTTCGTCTCGAACGA9340.10911176713165724No Hit
TGCTAGTGAAGGCGTA9300.10864447904972295No Hit
TCGGACCGATAGGCGA9290.10852765702923939No Hit
CACATTACTGATGGAC9210.1075930808653708No Hit
AGCCAATTGTTATGGC9160.10700897076295293No Hit
CGAGTCTCTTGGAACG9080.10607439459908434No Hit
TGAGGGATGTACGTTG9040.10560710651715005No Hit
ATGTCCAGACCGCTAG9020.1053734624761829No Hit
ACCTCAGCTAATCCGT8980.10490617439424862No Hit
TGTAAGCACGCGCTGT8950.1045557083327979No Hit
GCCATTCACCGGAAGT8910.10408842025086361No Hit
AGGCGATGACCTGCGA8840.1032706661074786No Hit
TGCGCAGACATCAGGG8780.10256973398457717No Hit
ACCAAGTTGAAGCTAA8710.10175197984119214No Hit
CATAGTCGAAGATTCC8670.10128469175925785No Hit
CTAACAGTGCATGATC8660.10116786973877429No Hit
TAGTACCGAACGAGCA8640.10093422569780713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)