FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3954581
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGTGAACGTAA205260.5190436104355935No Hit
CGACCAGACGCGCTGT192610.48705539221475047No Hit
TAGCTTTACCCTATCC183340.4636142236054843No Hit
CCAGGACTGTAACTCG140530.3553600242351844No Hit
ATCTGTACTGGGACTA132800.33581307349628187No Hit
GTTCTCGACCGTAGAG123590.31252362766118585No Hit
CTTCCTTTGGCTCAGC122390.3094891721777857No Hit
CGATCAAACCCGGCTA116120.29363414227702No Hit
AGACCATTGAATCCAC103600.2619746567335452No Hit
GGGGGGGGGGGGGGGG102830.2600275477983635No Hit
TGCTGCTACTTTCTTG100340.2537310526703082No Hit
GGCAACAGAATAGCAA99880.2525678447350048No Hit
ACAGCCGACTTGCGTA94420.23876107228553417No Hit
AACTTACCTCACCTAA85770.21688770567602483No Hit
CGCGTTGGAGCGGTTC81840.2069498639678894No Hit
ATCGAAAGATTTCCGC77300.19546950738902552No Hit
CCCTGAACTAGCTTTG76770.1941292895505238No Hit
GTAACTGTGTGAAAGT75620.19122126971226533No Hit
AGTGGAGTGAGTTGAA74890.18937530929319693No Hit
CAATTCGGATAACGGG72300.1828259428748583No Hit
GGGTAGAACCAGTCTA67810.17147202194113612No Hit
TTCCTAAACTAATGCC65100.16461920997445748No Hit
AAGAACCCTTAACCCA64350.1627226752973324No Hit
TTTAGTCTGAGCAATA64040.161938774297454No Hit
CGCGATCGATATCTGG62400.15779168513680716No Hit
TCCGGGAACGTTCTCC62230.15736180394332547No Hit
TGGAACACTCCTAGTA60240.1523296652666869No Hit
GTGAAGCGATCCCAGG59790.15119174446041186No Hit
TGCAGATACTGCCACG58800.14868831868660676No Hit
CACATCCTGAGCCTAT56540.14297342752620315No Hit
TGATAGAGAGACTGTT56370.14254354633272148No Hit
AGTCCTGCTCGAACGA56040.14170907107478645No Hit
CTTTAACTGTCCGTAA55680.14079873442976637No Hit
CATAGGTACAAGGTGC55490.14031827897822804No Hit
GCAACTTGAAGGTGCA55440.14019184333308637No Hit
AGGTGTGGATCATGTG54990.1390539225268113No Hit
TGCAGTATGACACCGC54270.13723324923677124No Hit
TCTCAGAGATGCATGT53000.13402178385017277No Hit
TCAAAGCACTCTGCAA52910.13379419968891773No Hit
ATTTCTGACGGGTAGT52250.13212524917304766No Hit
CTATTGGGACTTGTGA52150.13187237788276432No Hit
CTTAGATCTTGTCACA52090.13172065510859432No Hit
TACGTTTACTGCACCG51140.12931837785090253No Hit
TGGCAATTGAGTGGTG50590.12792758575434415No Hit
ATGTACGTGTACTTTC50570.12787701149628747No Hit
TGAACTGTGAACGTAA50520.1277505758511458No Hit
GAGTGTTACACCTGCG50480.12764942733503246No Hit
AGAGTACCTTAATCGC50100.12668851643195575No Hit
TGAGGGATGTACGTTG49770.12585404117402071No Hit
GGTCTAAACTCCTACC49320.12471612036774565No Hit
GGTCGCATGGTACTAC48440.12249085301325223No Hit
TCTATGGGATGCTCAA48080.12158051636823218No Hit
CGAGTCTCTGCTACGC47320.11965869456207875No Hit
TTCTTAGACGTCCCGA47320.11965869456207875No Hit
GGGTGTGCTCGCAAAG47010.11887479356220039No Hit
GGGATGTGACTGGAAG46460.117484001465642No Hit
GCATGATACTTGCGTA46250.11695297175604696No Hit
TCCATTTGAAATCTGC45560.1152081598530919No Hit
TACTGTTTGCTTAAGC45320.11460126875641188No Hit
TCCGGGTGAACACCTA44700.11303346675665513No Hit
GTAATCTCTACACAAA44030.11133922911175673No Hit
GATGAATGACTCCTAC43950.11113693207953003No Hit
TTGCGGTCTTTTGGGT43790.11073233801507669No Hit
AGACTACTGGCCCTAG43610.11027716969256667No Hit
CTAGCTCACGAATAGT43450.10987257562811333No Hit
TCGGACCGATAGGCGA43360.1096449914668583No Hit
CATTCTATGCCTAGCG43250.10936683304754663No Hit
CTACAACGAATCTGTG43230.10931625878948996No Hit
CTTTCGTCTCGAACGA42940.10858293204766827No Hit
CACCAGGTGCGCCACA42920.1085323577896116No Hit
CGAGTCTCTTGGAACG42890.1084564964025266No Hit
ACCAAGTTGAAGCTAA42660.1078748924348749No Hit
NNNNNNNNNNNNNNNN42140.1065599617254015No Hit
AGCCAATTGTTATGGC42100.10645881320928816No Hit
CACATTACTGATGGAC42050.10633237756414649No Hit
GGCTGACTGAAACTGA41790.10567491220940979No Hit
TGTAAGCACGCGCTGT41740.10554847656426812No Hit
TGCGCAGACATCAGGG41490.10491629833855975No Hit
GGGCCTTGACTATCTT41350.10456227853216309No Hit
ATGTCCAGACCGCTAG41220.10423354585479473No Hit
CATAGTCGAAGATTCC40870.10334849633880303No Hit
CTAACAGTGCATGATC40720.102969189403378No Hit
ATAGCCAACCGTTCTC40510.10243815969378299No Hit
TGCTAGTGAAGGCGTA40340.1020082785003013No Hit
ACGAGGATGAACTAGA39610.10016231808123288No Hit
GCCATTCACCGGAAGT39550.10001059530706288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)