FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088957
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT333361.0791992248516247No Hit
TGCTGCTACCGTTCTC309481.001891576995083No Hit
AAACGACCTCTTCAAG294790.9543350716762973No Hit
ATGGGTTACAATCGAA288500.9339722113321746No Hit
TATGCTTTGGACTGTT283790.9187243461142386No Hit
GCCTCTTGATCTGTGA275210.8909479801758328No Hit
AAAGCGGACTCGGGCT267110.8647255368074078No Hit
TCCAAAGTGCTCACGG253410.8203739967892075No Hit
TAGTGCATGAACCCGC242170.7839863099421585No Hit
TGTGGCTCTTACGATC228580.7399908771795787No Hit
TAACTCACTGGTGTCC227020.7349406288271413No Hit
GCAGGTCTGGCTGTTA224750.7275918700066074No Hit
GAAGTATGAACAGGTG212220.6870280162527351No Hit
TTCCCATCTCTTTGTT205630.6656939543023745No Hit
GAGCCTGACCGAGTTT197690.6399894851239432No Hit
TCACCTGACTCAATGC192840.6242883924897628No Hit
GGAACTACTACCAAGG191110.6186877965604571No Hit
TGAGAATGAGCAGTTT188230.6093642611405727No Hit
CTAGAGACTAATCTGC185660.6010443007138008No Hit
ATGTCCAGAGTCGGTC184870.5984868031507075No Hit
TTCGTCCGACCTGTGG177160.5735269218703918No Hit
TCGTATACTGCTAATC176940.5728147073591506No Hit
TCACACAGATTGGGAG169910.5500562163863078No Hit
ATTCCTAACTGCTACA169380.5483404268819541No Hit
TCCGCGAGAGAACAGC163820.5303408237796771No Hit
TGGCCTGGACCCGAGT163810.5303084503928025No Hit
ACTGATTCTCGGCTCA163310.5286897810490725No Hit
AGGTCGAGAAGGGAGG162800.527038738318468No Hit
TGGACCTCTAGGGAGG154700.500816294950043No Hit
CCATGATGATTCGGGC153570.4971581022332134No Hit
GCAATCATGGAACGTG151210.489517982930808No Hit
ACGAGGATGGAGCTGA140240.45400437752937317No Hit
TTCGATGGAAATCCTG139120.4503785581994181No Hit
AAGGTGCGAACAGATA132140.4277819341609482No Hit
TCCCAGACTGTCTTGA131500.42571003740097385No Hit
TTTCGTAACAGTACGG128500.41599802133859426No Hit
TCTCTCTACTCTGGAT125590.40657736575808595No Hit
TGGTCCTTGGAGAGGG125030.4047644560931084No Hit
TGCGGTGACCTTAGTG124600.40337240045750067No Hit
TCCTTCACTTGTCACA113670.36798828860356425No Hit
AGCCCAGTGAAAGTAC109660.35500656046685014No Hit
TGATATCCTCGATGTT108750.35206058226126163No Hit
AGCTGCTCTTGTCACA107830.34908223066879857No Hit
TTCGATGGACAGGTGA107820.349049857281924No Hit
TCGCCGAGAAGGTAAT107740.34879087018692717No Hit
AACTCCCTGCTTCACC104840.33940258799329354No Hit
CTTTCGGTGCTTTGTT104400.3379781589708112No Hit
AACCCTCACGCGGTTC102280.33111500095339624No Hit
ATGGCCGTGATACGCT101900.3298848122521615No Hit
CCCTTGCCTCCTACGG101450.3284280098428045No Hit
TCGGCTTACCTCCTAC94240.30508679790621884No Hit
GGGGGGGGGGGGGGGG94200.3049573043587204No Hit
GAAGTAATGAGTCTGT93620.3030796479199937No Hit
TGTTTGTTGCAAGTTC93000.30107249793376856No Hit
AAACACGCTGCAGTTT92500.29945382859003866No Hit
CTAACTGGAACCTTGA90750.29378848588698386No Hit
ACCCTTGTGGGCCATC89590.2900331730095304No Hit
TGGCCTTTGCGAAAGC89100.2884468770526751No Hit
TACAACGTGAGCAGGT88470.2864073536795753No Hit
AAATAGGACTCTATCT87690.2838822295033567No Hit
ACAGCCGACAAACATG84910.2748824279522182No Hit
CAATGTGTGGTTGACT84430.2733285053822374No Hit
CTTTCGGTGAGCCATT82880.26831063041667463No Hit
TCAAAGGCTCTTAGTG81700.264490570765472No Hit
GGACATTTGGCCGCTA79150.2562353571124493No Hit
TGAACTGTGTGCTGGC76850.24878947813129154No Hit
CAGCCGATGTAATAGG75660.2449370450932143No Hit
AGTGTTCGATTCGCCT74350.2406961314126419No Hit
GGCTTGCGAATACGCT69450.22483317184408846No Hit
GATAAGAACATTGGTC66450.21512115578170887No Hit
GTAAAGCCTGAAATCA65810.21304925902173455No Hit
GTGCTAATGGGAGTCA64800.20977954694740003No Hit
TTGGGAGGAATCTGTG64770.20968242678677623No Hit
TGTACTGGAAATGCTC62070.20094161233063457No Hit
CGACGTTCTTGCTACA61060.1976719002563001No Hit
GGAACATCTTTACGAC60960.19734816638755415No Hit
GGACCACTGGCTAGAG60040.19436981479509102No Hit
AGGGACCCTAATCCTG58790.1903231414357662No Hit
TCTTGAACTCGTACAT57840.1872476696826793No Hit
TAACGAGCTTACTTTC57220.1852405196964542No Hit
GGGTGAAACCGACTAT57170.18507865276208119No Hit
TACCACCTGAAACATG56020.18135571327150232No Hit
CGGATGAGAACAGGCA54490.17640258507968873No Hit
TACGTGTGAAACGCAG54430.1762083447584411No Hit
AGCTTGTGAACCGTGC53750.1740069544509684No Hit
CATGATGTGCCTAGTA53670.17374796735597162No Hit
AACTAGCACTGCCACG53160.17209692462536708No Hit
ACTTATCTGTCAGGTT51780.16762939723667244No Hit
ACGTGTTGATCTTTGT48670.15756127391867222No Hit
TCGGACCGAATGTCCA48420.15675193924680728No Hit
AACTACGACATTGCCA47700.15442105539183612No Hit
TGGTAGGGATGTGCTA47550.15393545458871716No Hit
GTAGATAGAGCTGAGA47530.15387070781496798No Hit
AACGAACCTGACACCC47060.15234915863186183No Hit
GTCCGAGTGGTCTTGA47050.1523167852449872No Hit
CTCGGATCTAGTACGG46310.14992115461626693No Hit
CGTCGTACTTGCGCCA45690.14791400463004178No Hit
TTTAGGGACTGGGCAA45660.14781688446941801No Hit
TACCACCTGTACTTTC45310.14668381592880703No Hit
GGTAGTTTGTCCAGCG44470.14396445143134073No Hit
GCCATAATGACCGTTA44070.1426695159563568No Hit
AGTTTGCGAGCAGGAT43950.1422810353138616No Hit
CTTTAACTGTTCGCCT43230.1399501514588905No Hit
CCTTCAGTGCAACGCC42940.13901132323952714No Hit
TGAGGAGTGGTCCCAG42550.13774876115141776No Hit
CAAAGACCTGCTTTGA39710.12855471927903173No Hit
CTTACATTGGAAGGCT39290.12719503703029858No Hit
AGAACGCGACACAGTT39000.12625620881093522No Hit
CGATCGGTGCGAGGAT38910.12596484832906382No Hit
GTCCGTTACGGATGGT38480.12457279269345609No Hit
TGCAATGACTCCGTAA38020.12308361689722454No Hit
CCGTTACTGTTGGTTG37710.12208004190411197No Hit
GAAAGTCCTTATCTGG37670.12195054835661358No Hit
GAACCATACTTTGGGT37510.12143257416661998No Hit
GGGATGTGATGCATGT37500.12140020077974539No Hit
GAGAAGAGATGCTACA37380.12101172013725021No Hit
CAGGCCATGTCGTAGT37370.12097934675037562No Hit
AACTACGACGCTGTTA37140.12023475885225984No Hit
GGCTTAAACTGCATCA35510.11495789679170025No Hit
GCTGGACACATTGGTC35370.11450466937545586No Hit
TCCTCATCTTTCTGGA35290.11424568228045907No Hit
ACAAGACCTAAGCTAA34640.11214141213361013No Hit
AATTCTGCTTAGCGAG34590.11197954519923715No Hit
TGTCTTCCTGAAATCA34260.1109112234323754No Hit
TCGTAGAACCGCACTG34070.11029612908175802No Hit
CCCACCTCTTGACCTA34030.11016663553425961No Hit
ATAGCCAACATCTGTG33520.10851559280365508No Hit
CCTGAGGACTGACTCA33030.10692929684679975No Hit
TCCGTAAGATTACGCA32930.10660556297805376No Hit
ATGGCCGTGGGCCAAA32240.10437179928370645No Hit
TATGGGTACCCTTTGC32160.10411281218870964No Hit
GAGGTTGGAGGAATAT31740.10275312993997651No Hit
AGGTGATCTGTTAGTC31280.10126395414374496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)