FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325831
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC152010.6535728520257921No Hit
CTCCTAGTGAACGTAA119070.5119460528301497No Hit
TAGCTTTACCCTATCC104880.45093560108193587No Hit
CGACCAGACGCGCTGT90820.39048408934269085No Hit
CCAGGACTGTAACTCG84420.36296704274730196No Hit
ATCTGTACTGGGACTA75440.32435718674314684No Hit
GTTCTCGACCGTAGAG75020.32255138056032445No Hit
CTTCCTTTGGCTCAGC71240.3062991249149229No Hit
CGATCAAACCCGGCTA70490.3030744710170257No Hit
NNNNNNNNNNNNNNNN66640.28652124767448706No Hit
TGCTGCTACTTTCTTG59650.2564674733460858No Hit
GGGGGGGGGGGGGGGG58880.25315682867757805No Hit
ACAGCCGACTTGCGTA55800.23991425000354713No Hit
GGCAACAGAATAGCAA54050.2323900575751205No Hit
AACTTACCTCACCTAA50730.21811558965376246No Hit
CGCGTTGGAGCGGTTC48020.20646384023602746No Hit
ATCGAAAGATTTCCGC46800.20121840322878146No Hit
GTAACTGTGTGAAAGT45480.19554301236848248No Hit
CCCTGAACTAGCTTTG44370.19077052459959473No Hit
AGTGGAGTGAGTTGAA43670.18776084762822406No Hit
CAATTCGGATAACGGG42610.18320333678586279No Hit
GGGTAGAACCAGTCTA40360.17352937509217137No Hit
AAGAACCCTTAACCCA39060.16793997500248298No Hit
TTCCTAAACTAATGCC38530.16566121958130234No Hit
GTGAAGCGATCCCAGG37250.16015781026222456No Hit
CGCGATCGATATCTGG37160.15977085179447692No Hit
TCCGGGAACGTTCTCC37070.15938389332672925No Hit
TGGAACACTCCTAGTA36660.15762108252921214No Hit
TTTAGTCTGAGCAATA36000.15478338709906267No Hit
TGCAGATACTGCCACG35070.15078481626567022No Hit
TGATAGAGAGACTGTT34830.1497529270183431No Hit
CACATCCTGAGCCTAT33640.14463647616701297No Hit
CTTTAACTGTCCGTAA33530.14416352692865475No Hit
GCAACTTGAAGGTGCA33270.14304564691071708No Hit
AGTCCTGCTCGAACGA33150.14252970228705353No Hit
TCTCAGAGATGCATGT32170.1383161545271346No Hit
TGCAGTATGACACCGC32060.13784320528877636No Hit
CATAGGTACAAGGTGC31640.13603739910595394No Hit
GAGTGTTACACCTGCG31500.13543546371167983No Hit
ATTTCTGACGGGTAGT31250.13436057907904744No Hit
CTATTGGGACTTGTGA31030.13341468060233094No Hit
AGGTGTGGATCATGTG30880.13276974982275153No Hit
TACGTTTACTGCACCG30620.13165186980481383No Hit
CTTAGATCTTGTCACA30360.13053398978687616No Hit
AGAGTACCTTAATCGC30350.1304909944015709No Hit
TCAAAGCACTCTGCAA30260.13010403593382322No Hit
ATGTACGTGTACTTTC29990.12894316053058025No Hit
TGGCAATTGAGTGGTG29880.12847021129222203No Hit
TGAACTGTGAACGTAA29790.12808325282447436No Hit
TGAGGGATGTACGTTG29690.1276532989714214No Hit
GGTCTAAACTCCTACC29040.12485859892657722No Hit
TTCTTAGACGTCCCGA28790.12378371429394483No Hit
CGAGTCTCTGCTACGC28660.123224774284976No Hit
GGTCGCATGGTACTAC28380.12202090349642772No Hit
GGGTGTGCTCGCAAAG28370.12197790811112244No Hit
TCTATGGGATGCTCAA28250.1214619634874589No Hit
GCATGATACTTGCGTA27560.11849528190139352No Hit
GGGATGTGACTGGAAG27510.11828030497486705No Hit
TCCATTTGAAATCTGC27260.11720542034223468No Hit
AGACTACTGGCCCTAG27130.11664648033326583No Hit
GTAATCTCTACACAAA26940.11582956801246523No Hit
CTACAACGAATCTGTG26800.11522763261819109No Hit
CACCAGGTGCGCCACA26660.11462569722391697No Hit
TACTGTTTGCTTAAGC26500.11393777105903223No Hit
CTCAATTTGGAATCTC26360.11333583566475809No Hit
TTGCGGTCTTTTGGGT26170.1125189233439575No Hit
TCGGACCGATAGGCGA26000.11178800179376747No Hit
TGTAAGCACGCGCTGT25980.1117020110231569No Hit
TCCGGGTGAACACCTA25910.11140104332601983No Hit
GGCTGACTGAAACTGA25910.11140104332601983No Hit
AGCCAATTGTTATGGC25830.11105708024357745No Hit
CTTTCGTCTCGAACGA25540.1098102140697239No Hit
CGAGTCTCTTGGAACG25540.1098102140697239No Hit
CTAGCTCACGAATAGT25370.10907929251953387No Hit
CACATTACTGATGGAC25330.10890731097831269No Hit
AGGCGATGACCTGCGA25290.10873532943709151No Hit
TGCTAGTGAAGGCGTA25290.10873532943709151No Hit
TGCGCAGACATCAGGG25250.10856334789587033No Hit
CATTCTATGCCTAGCG25180.10826238019873327No Hit
ACCAAGTTGAAGCTAA24850.10684353248365853No Hit
ATGTCCAGACCGCTAG24230.10417781859473021No Hit
CATAGTCGAAGATTCC24210.10409182782411965No Hit
TAGTACCGAACGAGCA23490.1009961600821384No Hit
GATATGAGACCAACGC23460.1008671739262225No Hit
GTCTAATCTAAGTTCC23420.10069519238500133No Hit
ATAGCCAACCGTTCTC23350.10039422468786426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)