FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3625756
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG354070597.65425472646257No Hit
TAATGGTG96480.26609622931052174No Hit
TATTGGTA84050.23181372381373705No Hit
TATTGGGG75790.20903226802906757No Hit
AATTGGTG67200.18534065723120915No Hit
TATTGGTC59290.16352451736961893No Hit
TATTGCTG54240.1495963876223331No Hit
TATTGGAG53300.1470038248574918No Hit
TATTGGTT48560.13393068921350473No Hit
TATTCGTG47180.1301245864310781No Hit
TATAGGTG44000.12135400175852981RNA PCR Primer, Index 39 (100% over 8bp)
TTTTGGTG41570.11465195120686554RNA PCR Primer, Index 28 (100% over 8bp)
TAGTGGTG38760.10690184336728671No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)