FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269548
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT576321.7626901333150635No Hit
GAAGCAGTGGAACCTT209690.6413424730268527No Hit
GTTCTTCCTAACGTAA168400.5150559037518335No Hit
GATACGTACCGCTCGA144550.4421100408986196No Hit
CGCTTAAGATAATAGG133400.40800746769889906No Hit
TGTCCCATGAGACAAT132710.40589708424528403No Hit
CATGGAGGATCGACAT131590.4024715342915901No Hit
AAAGCATGATCTTTGT120150.36748198833600243No Hit
TGAGCGCACAGGACAC119280.36482107006840087No Hit
TGTCGAAACTTCGCAA110530.33805896105516725No Hit
TAATTGCCTGTCTGGC109970.3363461860783203No Hit
GGAAGGACTAGGTAAT98060.2999191325528789No Hit
GGGGGGGGGGGGGGGG91610.2801916350516952No Hit
AATGATGGAAAGCTAA87730.2683245512835413No Hit
CATGTCAGAGGTTTGT84380.2580784866899033No Hit
TCGACCTTGTTTCGCC81660.24975929394521812No Hit
GTGAGATCTCGATTAC70150.21455565111752453No Hit
ATGGGTACTTGGCTGC66610.20372846644245626No Hit
AAACGACCTAGGGAGG64720.1979478508955978No Hit
CAGCTCACTGTTTACG61920.18938397601136306No Hit
TTGCGATGACATTCAG61910.18935339074391935No Hit
AGCCTACCTTAACTCG60880.1862031081972187No Hit
TGTTCCTCTTTATGGC59060.1806365895224661No Hit
GCTATGATGACCTGCG58650.17938259355727457No Hit
CAATGTGACCAACGCC54740.1674237539867896No Hit
CTGCCTTGACCATAAC54470.16659795176580983No Hit
CCTGTTGACCCTAGTA52680.1611231888933883No Hit
CATGCAAACGAAGGCT52240.15977743712586573No Hit
AGCTTGTGACTCACTC52120.15941041391654137No Hit
ATGCCGCGATGCCACG51570.1577282242071381No Hit
TAACCAGACATCACAC51390.15717768939315158No Hit
CACAGCTCTGGACTGA51260.15678008091638357No Hit
CATGATGTGTCATGTG51160.1564742282419466No Hit
ATGGGTACTTTCGGGC50920.15574018182329788No Hit
CTATTCAACCGAACGA49640.15182526759050485No Hit
TGGGCCATGCGTTCTC49190.15044893055553857No Hit
TTGTTTGTGATACGCT48810.14928669039267814No Hit
CCGCTATTGTTCGCAA47580.14552470249710356No Hit
ATTAGCGTGGTCGGTC47470.14518826455522293No Hit
GGGTCGTACCGCTCGA47270.14457655920634901No Hit
AGAACGCGAGGAATAT47050.1439036833225877No Hit
CATTAGTCTATTCTGG46760.14301671056672055No Hit
CACTATACTACGCCAC46000.14069223024099967No Hit
GGGCTGACTAATACAG44810.1370525834151999No Hit
AACGTCCCTTACAGAA43630.13344352185684383No Hit
CCCAATGCTGGAATGC43150.13197542901954643No Hit
TTGACGTGACCTCAAA43060.13170016161255318No Hit
ATAGCCAACATGGCCC42940.13133313840322883No Hit
CCTACGGGATGGAACG42530.1300791424380373No Hit
ATAGGAAACAGACTTC42480.12992621610081884No Hit
CTGCCATTGCACAGTT42250.12922275494961383No Hit
GGTAGCCTGTGCCACG42220.12913099914728274No Hit
AACCAACACCGGAATG42140.12888631700773318No Hit
AGCTCGGACCTTAAGC42130.12885573174028947No Hit
CTGATTACTGCTTTGA42100.12876397593795838No Hit
TTGTAACCTCATCCTC41860.12802992951930972No Hit
TGGAGTTACAAGTCTC41860.12802992951930972No Hit
TCAGGCTCTTAAAGCA40920.12515491437960233No Hit
GGCTTGCGAGTACGTT40270.12316687199576211No Hit
CGCTTACTGGCCACGT39760.12160702335613363No Hit
TGTTTGTTGCGCTCGA39580.12105648854214711No Hit
GCAGATACTCGGCTCA38930.11906844615830689No Hit
CGATCAAACCGATCGC38890.11894610508853211No Hit
TCCAAAGTGAAGGCCA38670.11827322920477082No Hit
ATCTTACTGCGCACTG38550.11790620599544646No Hit
TTCTAACACTACGTTG38260.1170192332395793No Hit
CGCTGAGTGTTAGCTG38100.11652986896048016No Hit
TGGGTGCACTTCTGAG37690.11527587299528863No Hit
CGCTTCCCTGCGCTGT37690.11527587299528863No Hit
CTCGGATCTTTCTGAG37490.11466416764641474No Hit
CATGTTACTAACGTGG37200.11377719489054755No Hit
GTAAGAAACGACAAAT36900.11285963686723668No Hit
CGAGTCTCTTACTTTC36860.1127372957974619No Hit
CTTAGGCCTTATGACC36850.11270671053001823No Hit
GCGACGTTGCTTAGTG36500.11163622616948887No Hit
GCGTCGATGCTTGAAC36440.11145271456482671No Hit
GATACCATGTCTGGAT36430.111422129297383No Hit
CCTTCAGTGTAGCTTG36280.11096335028572757No Hit
TACGCCTTGGGTTGAG35720.1092505753088806No Hit
AGTGGTACTCAACGCC35630.10897530790188736No Hit
AACAGCTACTCGAGAG35590.10885296683211257No Hit
CCTCCCTTGTTTCGCC35220.10772131193669585No Hit
GAAAGTAACCCTGCGA35190.10762955613436474No Hit
AGCCGGTACGCGCAAA35170.10756838559947736No Hit
AGAACAATGAGTCGGA35140.10747662979714628No Hit
ACCTGCTTGCCAAACT34940.10686492444827236No Hit
TGAGATAGACTCATCC34910.10677316864594127No Hit
ATTGAGAGATGATCAC34700.10613087802962366No Hit
ACCTCCATGGAATTGA34120.10435693251788931No Hit
AACCACTGAAAACATG34060.10417342091322715No Hit
TATCTCCCTGGAATTA34000.10398990930856498No Hit
TACAACCGATTCCATT33950.1038369829713465No Hit
GGTCAGAGAACCAGTT33880.10362288609924063No Hit
TTCACGCTGTGGGCAA33680.10301118075036672No Hit
ATCGCAATGTAACTCG33660.10295001021547934No Hit
GTTGCTAGACGCTCGA33650.10291942494803563No Hit
AGGTTACTGGTCCCGA33050.1010843089014139No Hit
TTCTATGACCAACGCC32880.10056435935487107No Hit
TCCGGGATGCGAGTTT32870.10053377408742739No Hit
ATTCCTAACATTGCCA32860.10050318881998369No Hit
GTCCTGACTTTACGAC32810.1003502624827652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)