FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20266419
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG30350.08.94844155
TCTATAC16550.08.6279723
ATACCGT5550.08.576116
GTACTAG13150.08.4115371
CTTAGAC19550.08.3064943
GTATACG4900.08.28659251
TATACTG22700.08.2021965
TAATACT25250.07.9282824
TACACTG33550.07.9280335
GTATTAG21300.07.888161
GTGTAGG30050.07.87437871
GTCCTAG27800.07.85694741
ATACTGT32050.07.77492766
CTAGTAC12100.07.75168283
GTTCTAG25750.07.6124571
TGTACCG8900.07.550155
CTAATAC22350.07.5164063
GTATAGG16850.07.4785371
ATAATAC21200.07.4618943
CGTCAGA27400.07.46064421
Produced by FastQC (version 0.11.5)