FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810590
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC50370.6213992277230166No Hit
CTCCTAGTGAACGTAA42230.5209785464908276No Hit
CGACCAGACGCGCTGT41110.5071614503016322No Hit
TAGCTTTACCCTATCC38480.4747159476430748No Hit
CCAGGACTGTAACTCG29700.3663997828742027No Hit
ATCTGTACTGGGACTA27450.338642223565551No Hit
GTTCTCGACCGTAGAG25880.3192736155146252No Hit
CTTCCTTTGGCTCAGC25410.31347536979237345No Hit
AGACCATTGAATCCAC25230.31125476504768135No Hit
CGATCAAACCCGGCTA24580.3032359145807375No Hit
GGGGGGGGGGGGGGGG24530.3026190799294341No Hit
TGCTGCTACTTTCTTG21460.2647454323394071No Hit
ACAGCCGACTTGCGTA20110.24809089675421606No Hit
AACTTACCTCACCTAA17500.21589212795618007No Hit
CGCGTTGGAGCGGTTC17440.215151926374616No Hit
ATCGAAAGATTTCCGC16830.20762654362871485No Hit
GTAACTGTGTGAAAGT16390.20219839869724524No Hit
CCCTGAACTAGCTTTG16090.198497390789425No Hit
GGCAACAGAATAGCAA15890.1960300521842115No Hit
AGTGGAGTGAGTTGAA15610.19257577813691262No Hit
CAATTCGGATAACGGG15420.1902318064619598No Hit
TTCCTAAACTAATGCC13920.17172676692285865No Hit
AAGAACCCTTAACCCA13910.17160339999259797No Hit
GGGTAGAACCAGTCTA13440.16580515427034628No Hit
TTTAGTCTGAGCAATA12720.15692273529157774No Hit
TGCAGATACTGCCACG12650.156059166779753No Hit
CGCGATCGATATCTGG12540.15470213054688559No Hit
TGATAGAGAGACTGTT12430.1533450943140182No Hit
TCCGGGAACGTTCTCC12380.1527282596627148No Hit
GTGAAGCGATCCCAGG12290.15161795729036875No Hit
TGGAACACTCCTAGTA12250.15112448956932603No Hit
CTTTAACTGTCCGTAA11970.14767021552202717No Hit
CACATCCTGAGCCTAT11560.14261217138133953No Hit
CTATTGGGACTTGTGA11460.14137850207873276No Hit
TCTCAGAGATGCATGT11440.1411317682182114No Hit
AGGTGTGGATCATGTG11410.1407616674274294No Hit
AGTCCTGCTCGAACGA11370.1402681997063867No Hit
CATAGGTACAAGGTGC11260.13891116347351928No Hit
ATTTCTGACGGGTAGT11180.1379242280314339No Hit
AGAGTACCTTAATCGC11000.13570362328674174No Hit
TACGTTTACTGCACCG10920.13471668784465637No Hit
TGCAGTATGACACCGC10860.1339764862630923No Hit
CTTAGATCTTGTCACA10830.1336063854723103No Hit
GGTCTAAACTCCTACC10700.13200261537892152No Hit
GAGTGTTACACCTGCG10610.13089231300657547No Hit
GCAACTTGAAGGTGCA10440.128795075192144No Hit
ATGTACGTGTACTTTC10440.128795075192144No Hit
TGAGGGATGTACGTTG10390.12817824054084062No Hit
CGAGTCTCTGCTACGC10380.12805487361057993No Hit
TGGCAATTGAGTGGTG10370.12793150668031927No Hit
TCTATGGGATGCTCAA10220.12608100272640915No Hit
TGAACTGTGAACGTAA10170.1254641680751058No Hit
TTCTTAGACGTCCCGA10070.12423049877249905No Hit
GGTCGCATGGTACTAC9970.1229968294698923No Hit
GGGATGTGACTGGAAG9940.12262672867911029No Hit
AGACTACTGGCCCTAG9830.12126969244624286No Hit
CTCAATTTGGAATCTC9810.12102295858572151No Hit
CACCAGGTGCGCCACA9790.12077622472520017No Hit
TCAAAGCACTCTGCAA9760.12040612393441814No Hit
ACCTCAGCTAATCCGT9740.1201593900738968No Hit
TCCATTTGAAATCTGC9690.11954255542259343No Hit
GGGTGTGCTCGCAAAG9480.11695184988711926No Hit
TTGCGGTCTTTTGGGT9440.11645838216607655No Hit
TCCGGGTGAACACCTA9370.11559481365425184No Hit
TGCGCAGACATCAGGG9220.11374430970034173No Hit
TACTGTTTGCTTAAGC9200.11349757583982037No Hit
TCGGACCGATAGGCGA9140.11275737425825634No Hit
GTAATCTCTACACAAA9110.1123872734674743No Hit
CTTTCGTCTCGAACGA9080.11201717267669228No Hit
CGAGTCTCTTGGAACG9050.11164707188591028No Hit
CTAGCTCACGAATAGT9020.11127697109512824No Hit
CTACAACGAATCTGTG9000.1110302372346069No Hit
GGGCCTTGACTATCTT8950.11041340258330352No Hit
TGCTAGTGAAGGCGTA8830.10893299942017544No Hit
CATTCTATGCCTAGCG8780.10831616476887206No Hit
AGGCGATGACCTGCGA8670.10695912853600463No Hit
AGCCAATTGTTATGGC8670.10695912853600463No Hit
ATGTCCAGACCGCTAG8600.10609556002417991No Hit
ACCAAGTTGAAGCTAA8500.10486189072157316No Hit
TGTAAGCACGCGCTGT8490.1047385237913125No Hit
CTAACAGTGCATGATC8430.10399832220974844No Hit
GTATTCACTGCTCCTT8410.1037515883492271No Hit
ATAGCCAACCGTTCTC8400.10362822141896644No Hit
CACATTACTGATGGAC8350.10301138676766307No Hit
AGCTTGTGATTGCGTA8210.10128424974401364No Hit
AGTTTGCGAAACGAAT8130.10029731430192823No Hit
ACTCAGAACGGAATTA8110.10005058044140688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)