FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959718
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT533711.803246120069547No Hit
GAAGCAGTGGAACCTT161540.5457952413033944No Hit
GTTCTTCCTAACGTAA155820.5264690757700564No Hit
CGCTTAAGATAATAGG123370.41683025207131225No Hit
CATGGAGGATCGACAT121110.4091943894654828No Hit
TGTCCCATGAGACAAT117730.3977743825594195No Hit
AAAGCATGATCTTTGT114160.3857124226024236No Hit
TGTCGAAACTTCGCAA102600.3466546475035797No Hit
TAATTGCCTGTCTGGC100450.33939044192723766No Hit
TGAGCGCACAGGACAC97590.32972735916056867No Hit
GATACGTACCGCTCGA94780.320233211407303No Hit
GGGGGGGGGGGGGGGG92150.3113472297022892No Hit
GGAAGGACTAGGTAAT89280.30165035993293954No Hit
CATGTCAGAGGTTTGT78980.26684974717185894No Hit
AATGATGGAAAGCTAA78310.2645860179922547No Hit
TCGACCTTGTTTCGCC72150.24377322434096763No Hit
GTGAGATCTCGATTAC66090.22329830071648715No Hit
ATGGGTACTTGGCTGC61210.2068102434083247No Hit
GCTATGATGACCTGCG61190.2067426694029634No Hit
CAGCTCACTGTTTACG58100.1963024855746392No Hit
AAACGACCTAGGGAGG56850.1920791102395566No Hit
TTGCGATGACATTCAG56080.18947751103314572No Hit
AGCCTACCTTAACTCG54790.18511898768734047No Hit
TGTTCCTCTTTATGGC53930.18221330545680367No Hit
CTGCCTTGACCATAAC50540.17075951154805966No Hit
CAATGTGACCAACGCC49820.16832684735505207No Hit
CATGCAAACGAAGGCT48830.16498193408966663No Hit
TAACCAGACATCACAC48300.1631912229475916No Hit
CATGATGTGTCATGTG47560.16069098474922272No Hit
CACAGCTCTGGACTGA47520.1605558367385001No Hit
CCTGTTGACCCTAGTA47390.1601166057036515No Hit
AGCTTGTGACTCACTC47180.15940707864735762No Hit
ATGGGTACTTTCGGGC46800.15812317254549252No Hit
ATGCCGCGATGCCACG46580.15737985848651798No Hit
CTATTCAACCGAACGA46440.15690684044898873No Hit
CATTAGTCTATTCTGG45330.15315648315143537No Hit
TTGTTTGTGATACGCT44780.15129819800399905No Hit
AGAACGCGAGGAATAT44340.14981156988604996No Hit
ATTAGCGTGGTCGGTC43060.14548683354292538No Hit
GCATCTCTGAGCCTAT42230.14268251232043053No Hit
CACTATACTACGCCAC42030.1420067722668173No Hit
CCGCTATTGTTCGCAA40700.13751310091028943No Hit
GCAGATACTCGGCTCA40110.13551966775213045No Hit
CCCAATGCTGGAATGC40100.13548588074944978No Hit
AGCTCGGACCTTAAGC39830.13457363167707195No Hit
ATAGGAAACAGACTTC39690.1341006136395427No Hit
GGGATCAGATGAGTAA39670.13403303963418137No Hit
AACGTCCCTTACAGAA39530.13356002159665212No Hit
GGGTCGTACCGCTCGA39520.13352623459397145No Hit
AACCAACACCGGAATG39410.1331545775644842No Hit
CTGCCATTGCACAGTT38180.1289987762347629No Hit
CCTACGGGATGGAACG38040.12852575819723366No Hit
TGGAGTTACAAGTCTC37740.12751214811681383No Hit
TTGTAACCTCATCCTC37700.12737700010609118No Hit
CTGATTACTGCTTTGA37430.12646475103371335No Hit
TTGACGTGACCTCAAA37280.12595794599350343No Hit
CGCTTACTGGCCACGT36800.12433616986483173No Hit
ATAGCCAACATGGCCC36580.12359285580585719No Hit
TCAGGCTCTTAAAGCA36570.12355906880317652No Hit
TGGGCCATGCGTTCTC36150.12214001469058877No Hit
CGATCAAACCGATCGC35810.12099125659944629No Hit
TGTTTGTTGCGCTCGA35660.12048445155923637No Hit
ATCTTACTGCGCACTG35510.11997764651902648No Hit
GATACCATGTCTGGAT35230.11903161044396797No Hit
CTCGGATCTTTCTGAG35140.11872752741984202No Hit
CGCTGAGTGTTAGCTG35130.11869374041716135No Hit
CATGTTACTAACGTGG34910.11795042635818682No Hit
TCCAAAGTGAAGGCCA34790.1175449823260189No Hit
TTCTAACACTACGTTG34570.11680166826704436No Hit
CGCTTCCCTGCGCTGT34420.11629486322683445No Hit
TGGGTGCACTTCTGAG33880.11447036508207876No Hit
TACAACCGATTCCATT33640.11365947701774291No Hit
CGAGTCTCTTACTTTC33520.11325403298557497No Hit
CTTAGGCCTTATGACC33270.11240935791855845No Hit
ATTGAGAGATGATCAC33070.11173361786494525No Hit
CCTCCCTTGTTTCGCC32790.11078758178988675No Hit
AGTGGTACTCAACGCC32720.11055107277112212No Hit
GTAAGAAACGACAAAT32710.11051728576844146No Hit
GCGTCGATGCTTGAAC32570.1100442677309122No Hit
TATCTCCCTGGAATTA32490.10977397170946691No Hit
GCGACAAGAAAGGCTG32100.10845627860492114No Hit
TGAGATAGACTCATCC31980.1080508345727532No Hit
ATCGCAATGTAACTCG31950.10794947356471123No Hit
AACCACTGAAAACATG31950.10794947356471123No Hit
AACAGCTACTCGAGAG31870.10767917754326593No Hit
TACGCCTTGGGTTGAG31740.10723994650841735No Hit
GCATATCGAGCCGCTA31660.10696965048697206No Hit
CCTTCAGTGTAGCTTG31530.10653041945212348No Hit
GCATAGTACGCTCCTT31480.10636148443872018No Hit
TGGTCCTTGTTTCTGT31470.10632769743603952No Hit
CATGTCAGAACAGGTG31390.10605740141459423No Hit
AGAACAATGAGTCGGA31140.10521272634757771No Hit
GTGTACTGAATCACAC30850.10423290326983856No Hit
GGTCAGAGAACCAGTT30770.10396260724839326No Hit
ACCTCCATGGAATTGA30270.10227325711436022No Hit
AGGTTACTGGTCCCGA30160.10190160008487295No Hit
GGTAGCCTGTGCCACG29990.10132722103930172No Hit
AGAACATGAAAGCTAA29800.10068526798836916No Hit
AGCCGGTACGCGCAAA29620.10007710194011726No Hit
TTCACGCTGTGGGCAA29610.1000433149374366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)