FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3214006
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG314087197.72449086902762No Hit
GAGAAGGA195850.6093641393326583No Hit
GAGCAGGG87230.271405840561592No Hit
GAGAAGGC46590.1449592813454611TruSeq Adapter, Index 20 (100% over 8bp)
GAGAACGG42980.13372719279304393No Hit
GAGAAGCG40610.12635321776001662No Hit
GAGAAGTG38340.11929038091403688No Hit
GACAAGGG33580.10448020321057272No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)