FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4586580
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGTGAACGTAA242830.5294358759685867No Hit
GATGAATGACTCCTAC231160.5039920812457213No Hit
CGACCAGACGCGCTGT227510.49603408203934085No Hit
TAGCTTTACCCTATCC212710.4637660304627849No Hit
CCAGGACTGTAACTCG168210.36674384835759977No Hit
ATCTGTACTGGGACTA154020.3358057637716992No Hit
CTTCCTTTGGCTCAGC143500.31286928386728236No Hit
GTTCTCGACCGTAGAG137370.29950420574807374No Hit
CGATCAAACCCGGCTA136860.2983922661329357No Hit
AGACCATTGAATCCAC126720.2762842902554845No Hit
GGGGGGGGGGGGGGGG120550.2628320011860689No Hit
TGCTGCTACTTTCTTG118630.25864587557613733No Hit
ACAGCCGACTTGCGTA109490.23871817345385887No Hit
AACTTACCTCACCTAA100330.21874686585647693No Hit
CGCGTTGGAGCGGTTC95010.2071478094789582No Hit
ATCGAAAGATTTCCGC92520.20171892782857814No Hit
CCCTGAACTAGCTTTG89830.1958539914271636No Hit
AGTGGAGTGAGTTGAA88870.1937609286221978No Hit
CAATTCGGATAACGGG84000.18314299543450674No Hit
GTAACTGTGTGAAAGT83360.18174762023119623No Hit
AAGAACCCTTAACCCA78240.170584618604712No Hit
TTCCTAAACTAATGCC76460.1667037313205046No Hit
GGGTAGAACCAGTCTA75590.16480689315350436No Hit
TTTAGTCTGAGCAATA74290.16197253727177985No Hit
CGCGATCGATATCTGG73310.1598358689917106No Hit
TCCGGGAACGTTCTCC72020.15702331584753781No Hit
TGGAACACTCCTAGTA71870.1566962747842619No Hit
GTGAAGCGATCCCAGG71820.15658726109650328No Hit
TGCAGATACTGCCACG70030.15268457107474415No Hit
CTTTAACTGTCCGTAA67810.14784436333826076No Hit
AGTCCTGCTCGAACGA67050.1461873552843295No Hit
TGATAGAGAGACTGTT66640.1452934430447087No Hit
GGCAACAGAATAGCAA66430.14483558555612241No Hit
CACATCCTGAGCCTAT65550.14291694465157045No Hit
AGGTGTGGATCATGTG64590.14082388184660466No Hit
TGCAGTATGACACCGC64200.1399735750820873No Hit
TCTCAGAGATGCATGT63490.13842558071591468No Hit
ATTTCTGACGGGTAGT63430.13829476429060433No Hit
CATAGGTACAAGGTGC62940.1372264301505697No Hit
CTATTGGGACTTGTGA62110.13541680293377636No Hit
TCAAAGCACTCTGCAA61500.134086835943121No Hit
CTTAGATCTTGTCACA60460.1318193512377414No Hit
TACGTTTACTGCACCG59950.13070741162260335No Hit
TGGCAATTGAGTGGTG59770.13031496234667225No Hit
ATGTACGTGTACTTTC58420.12737159277718912No Hit
TCTATGGGATGCTCAA57710.12582359841101648No Hit
CGAGTCTCTGCTACGC57650.12569278198570613No Hit
TTCTTAGACGTCCCGA57350.12503869985915433No Hit
GCAACTTGAAGGTGCA57220.12475526427098187No Hit
AGAGTACCTTAATCGC57120.12453723689546459No Hit
TGAGGGATGTACGTTG56940.12414478761953351No Hit
TGAACTGTGAACGTAA56930.12412298488198177No Hit
TCCATTTGAAATCTGC54780.11943539630836049No Hit
TACTGTTTGCTTAAGC53800.11729872802829122No Hit
CACCAGGTGCGCCACA53410.11644842126377387No Hit
GGTCTAAACTCCTACC53330.11627399936336007No Hit
GGGTGTGCTCGCAAAG53120.11581614187477379No Hit
CTACAACGAATCTGTG52540.11455158309677364No Hit
CTCAATTTGGAATCTC52320.11407192287063563No Hit
TCCGGGTGAACACCTA52180.11376668454491147No Hit
CTAGCTCACGAATAGT51920.11319981336856656No Hit
TTGCGGTCTTTTGGGT51820.11298178599304928No Hit
CATTCTATGCCTAGCG51800.11293818051794582No Hit
ACCAAGTTGAAGCTAA51060.11132477793911805No Hit
CGAGTCTCTTGGAACG50720.11058348486235933No Hit
CTTTCGTCTCGAACGA50650.11043086569949723No Hit
TCGGACCGATAGGCGA50600.1103218520117386No Hit
NNNNNNNNNNNNNNNN50160.10936253155946261No Hit
GGGATGTGACTGGAAG49560.10805436730635899No Hit
CACATTACTGATGGAC49360.10761831255532445No Hit
TGCTAGTGAAGGCGTA49220.10731307422960026No Hit
AGCCAATTGTTATGGC49190.10724766601694509No Hit
TGCGCAGACATCAGGG48760.10631014830222082No Hit
GAGTGTTACACCTGCG48630.10602671271404839No Hit
TGTAAGCACGCGCTGT48510.10576507986342766No Hit
ATGTCCAGACCGCTAG48210.10511099773687584No Hit
TAGTACCGAACGAGCA48170.10502378678666895No Hit
AGGCGATGACCTGCGA47960.10456592929808267No Hit
GGTCGCATGGTACTAC47770.10415167728459986No Hit
ACCTCAGCTAATCCGT47490.1035412006331515No Hit
AGACTACTGGCCCTAG47460.1034757924204963No Hit
CTAACAGTGCATGATC47370.10327956778253078No Hit
GTAATCTCTACACAAA47200.10290892124415142No Hit
CATAGTCGAAGATTCC46880.10221123364249617No Hit
TCATCATTGGACTCGC46250.10083766117673736No Hit
AAAGCCTTGAACCAAA46040.10037980368815108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)