FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4440854
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT431344297.13091220742676No Hit
CTAGCTGA250060.563089892169389No Hit
CTAGCAGT89770.20214580348734723No Hit
CAAGCTGT83230.18741890636350575No Hit
CTAGCTAT71500.16100506794413866RNA PCR Primer, Index 38 (100% over 8bp)
CTAGATGT71290.16053218592640064No Hit
CTAGCTGG69530.15656898425392954No Hit
CTAGCGGT66960.1507818090844689No Hit
CTAGCTCT66930.1507142545105063No Hit
CGAGCTGT65610.147741853256153No Hit
CTCGCTGT63960.14402635168821132No Hit
CTACCTGT59220.13335272900212436No Hit
ATAGCTGT52130.11738733135563564No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)