FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16705150
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG20300.08.5556375
CTAGGAC24400.08.4382873
ATACCGT3702.564775E-108.3281596
CTAGTAC9650.08.2732153
TACACTG26950.08.1595765
TAGACTG21100.07.96572975
TTATACT21200.07.92815544
CTAGAAC25100.07.8681423
TATACGG3105.1376264E-77.6809335
TCTATAC16450.07.57793473
ACGAGAC10650.07.492056422
CTAGACT20300.07.45168454
GTGTTAG23700.07.4455971
TATACAC27850.07.44326163
AGGACCG14900.07.4262165
CTACACT25550.07.40064574
GTCTAGG23300.07.39309931
TAGGACC17100.07.37179334
TAGACAG31450.07.17021235
CCTAGAC21750.07.1480983
Produced by FastQC (version 0.11.5)