FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16705150
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA35700.09.7414621
TAGACTG22850.08.9438575
GTATAAG27700.08.796343
GTACCGT4850.08.6579616
TGTATAA32000.08.4515052
GTATTAG15950.08.0005781
TACACTG25600.07.9284135
TACACCG6050.07.86650375
TAGGACT22250.07.80262854
CTAGGAC24700.07.76705073
GTCTAGG25550.07.73871471
GTGTAGG22550.07.71109061
TAGACAG30650.07.6725025
TCTAGAC18950.07.5374333
CTAATAC15050.07.5366983
CTGATCG5501.8189894E-127.3803229
GTACTAG11500.07.31634331
ATACCGT3651.1965494E-77.2861296
TACACAG35050.07.2684465
GTATAAT17050.07.2376731
Produced by FastQC (version 0.11.5)