FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118611
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT333501.0693863389823226No Hit
TGCTGCTACCGTTCTC307260.9852463163889309No Hit
AAACGACCTCTTCAAG303860.9743440268760676No Hit
ATGGGTTACAATCGAA295460.9474089586678172No Hit
TATGCTTTGGACTGTT285450.9153113357196522No Hit
GCCTCTTGATCTGTGA275870.8845925317392903No Hit
AAAGCGGACTCGGGCT267050.8563107101206274No Hit
TCCAAAGTGCTCACGG254090.814753747742184No Hit
GCAGGTCTGGCTGTTA244550.7841632059913852No Hit
TAGTGCATGAACCCGC241980.7759223577419563No Hit
GAAGTATGAACAGGTG232900.7468068316311333No Hit
TAACTCACTGGTGTCC230020.7375719511025902No Hit
TGTGGCTCTTACGATC227280.7287859883775181No Hit
TTCCCATCTCTTTGTT204220.6548428130343925No Hit
GAGCCTGACCGAGTTT197960.6347697741077679No Hit
TCACCTGACTCAATGC196240.6292544982365547No Hit
GGAACTACTACCAAGG189700.6082836237029883No Hit
TGAGAATGAGCAGTTT188550.6045960846030493No Hit
CTAGAGACTAATCTGC183160.5873127491694219No Hit
ATGTCCAGAGTCGGTC182790.5861263235459633No Hit
GAAGTAATGAGTCTGT181880.5832083578234027No Hit
TCGTATACTGCTAATC179250.5747751162296292No Hit
TTCGTCCGACCTGTGG174330.5589988619933682No Hit
TGCGGTGACCTTAGTG173370.5559205684838538No Hit
TCACACAGATTGGGAG173330.5557923062542908No Hit
ATTCCTAACTGCTACA170120.5454992623318522No Hit
TCCGCGAGAGAACAGC166760.5347252350485521No Hit
AGGTCGAGAAGGGAGG166190.532897498277278No Hit
ACTGATTCTCGGCTCA166070.5325127115885887No Hit
TGGCCTGGACCCGAGT160530.5147483927940997No Hit
TGGACCTCTAGGGAGG159880.5126641315636994No Hit
CCATGATGATTCGGGC155850.49974171193521727No Hit
GCAATCATGGAACGTG143300.45949943740979554No Hit
TTCGATGGAAATCCTG139680.4478917056343352No Hit
ACGAGGATGGAGCTGA137030.43939433292578006No Hit
AAGGTGCGAACAGATA134290.43060837020070797No Hit
TTTCGTAACAGTACGG131610.42201480081998044No Hit
TCCCAGACTGTCTTGA131440.4214696863443373No Hit
TGGTCCTTGGAGAGGG128510.4120744780288404No Hit
TCTCTCTACTCTGGAT127380.40845107004368286No Hit
GCGGTTCGAGTACGTT120370.38597311431275017No Hit
TCCTTCACTTGTCACA112210.3598076194818783No Hit
TCGCCGAGAAGGTAAT111780.358428800514075No Hit
TTCGATGGACAGGTGA110540.354452671397619No Hit
TGATATCCTCGATGTT108990.349482510002049No Hit
AGCCCAGTGAAAGTAC107610.34505746308212215No Hit
AACTCCCTGCTTCACC106440.34130579286740154No Hit
AGCTGCTCTTGTCACA105940.3397025149978628No Hit
CTTTCGGTGCTTTGTT105610.3386443516039673No Hit
CCCTTGCCTCCTACGG99780.31995013164514585No Hit
ATGGCCGTGATACGCT99430.3188278371364688No Hit
AACCCTCACGCGGTTC98590.3161343303156437No Hit
TCGGCTTACCTCCTAC97710.3133125612652556No Hit
GGGGGGGGGGGGGGGG92760.29744011035682233No Hit
TGTTTGTTGCAAGTTC92180.29558030802815743No Hit
AAACACGCTGCAGTTT91630.2938167023716648No Hit
ACCCTTGTGGGCCATC90140.2890389343204394No Hit
CTAACTGGAACCTTGA89440.28679434530308523No Hit
TGGCCTTTGCGAAAGC87940.2819845116944691No Hit
TACAACGTGAGCAGGT86300.2767257602823821No Hit
AAATAGGACTCTATCT85250.27335887675635084No Hit
CAATGTGTGGTTGACT84470.27085776327987043No Hit
TCAAAGGCTCTTAGTG82590.26482943849040486No Hit
CTTTCGGTGAGCCATT81070.2599554737670072No Hit
TGAACTGTGTGCTGGC77830.2495662331723963No Hit
AGTGTTCGATTCGCCT74100.2376057802656375No Hit
CAGCCGATGTAATAGG73660.23619489574044344No Hit
GAGGTTGGAGGAATAT72470.23237909441094126No Hit
ACAGCCGACAAACATG68920.22099582153721642No Hit
GGCTTGCGAATACGCT67930.21782133135552975No Hit
GATAAGAACATTGGTC65990.21160061322171952No Hit
CGACGTTCTTGCTACA65470.2099332042373993No Hit
GTGCTAATGGGAGTCA65440.20983700756522697No Hit
GTAAAGCCTGAAATCA63330.2030711749557736No Hit
TTGGGAGGAATCTGTG62860.2015640937584072No Hit
TGTACTGGAAATGCTC61730.1979406857732497No Hit
GGACATTTGGCCGCTA59080.1894433130646945No Hit
AGGGACCCTAATCCTG58980.18912265749078677No Hit
TCTTGAACTCGTACAT58840.18867373968731593No Hit
GGGTGAAACCGACTAT58360.18713459293255877No Hit
GGAACATCTTTACGAC58160.18649328178474328No Hit
GGACCACTGGCTAGAG56470.18107420258570242No Hit
TAACGAGCTTACTTTC56020.17963125250311757No Hit
TACGTGTGAAACGCAG55620.1783486302074866No Hit
TACCACCTGAAACATG55460.1778355812892342No Hit
CGGATGAGAACAGGCA53780.17244856764758412No Hit
AGCTTGTGAACCGTGC53310.17094148645021773No Hit
AACTAGCACTGCCACG53180.17052463420413766No Hit
CATGATGTGCCTAGTA53080.17020397863022993No Hit
ACTTATCTGTCAGGTT53060.1701398475154484No Hit
ACGTGTTGATCTTTGT48520.15558208446003688No Hit
GTCCGAGTGGTCTTGA47780.15320923321311958No Hit
AACTACGACATTGCCA47550.15247172539313175No Hit
CGTCGTACTTGCGCCA47150.15118910309750078No Hit
TCGGACCGAATGTCCA46760.14993854635926057No Hit
CTCGGATCTAGTACGG46720.1498102841296975No Hit
AACGAACCTGACACCC46510.14913690742449123No Hit
TGGTAGGGATGTGCTA46350.14862385850623883No Hit
GTAGATAGAGCTGAGA45450.14573795834106915No Hit
GGTAGTTTGTCCAGCG44370.14227487814286552No Hit
AGTTTGCGAGCAGGAT44310.14208248479852087No Hit
TACCACCTGTACTTTC44160.14160150143765926No Hit
TTTAGGGACTGGGCAA44150.1415694358802685No Hit
CCTTCAGTGCAACGCC43870.1406716002733268No Hit
TGAGGAGTGGTCCCAG43020.13794602789511098No Hit
CTTTAACTGTTCGCCT42120.1350601277299413No Hit
GCCATAATGACCGTTA40520.12992963854741743No Hit
GTCCGTTACGGATGGT40120.12864701625178646No Hit
AGAACGCGACACAGTT40000.12826222956309719No Hit
GGGATGTGATGCATGT39590.1269475417100754No Hit
CAGGCCATGTCGTAGT38710.12412577265968727No Hit
CTTACATTGGAAGGCT38420.12319587149535483No Hit
CCGTTACTGTTGGTTG38240.12261869146232088No Hit
GAGAAGAGATGCTACA38020.12191324919972385No Hit
GAAAGTCCTTATCTGG37900.12152846251103455No Hit
CGATCGGTGCGAGGAT37870.12143226583886224No Hit
TGCAATGACTCCGTAA37010.11867462790325564No Hit
TCCTCATCTTTCTGGA36610.11739200560762467No Hit
GAACCATACTTTGGGT36470.11694308780415383No Hit
AACTACGACGCTGTTA36170.11598112108243061No Hit
CAAAGACCTGCTTTGA35870.11501915436070737No Hit
AATTCTGCTTAGCGAG34860.11178053306423918No Hit
GCTGGACACATTGGTC34700.11126748414598678No Hit
GAAGGACACCCAGCTG34600.11094682857207905No Hit
TCGTAGAACCGCACTG34080.10927941958775877No Hit
GCGTTTAGAAGTCTAC34030.10911909180080491No Hit
ATGGCCGTGGGCCAAA33760.108253321751254No Hit
TGTCTTCCTGAAATCA33740.10818919063647246No Hit
CCTGAGGACTGACTCA33570.10764407616082929No Hit
CCCACCTCTTGACCTA33300.10677830611127838No Hit
ACAAGACCTAAGCTAA33210.10648971609476142No Hit
GGCTTAAACTGCATCA32710.10488643822522271No Hit
AGGTGATCTGTTAGTC32050.1027701114374316No Hit
TCCGTAAGATTACGCA31780.1019043413878807No Hit
TATGGGTACCCTTTGC31760.10184021027309915No Hit
GCGTTAATGGTACGTT31650.10148748914180063No Hit
ATAGCCAACATCTGTG31530.10110270245311133No Hit
GACGAGTGATGCTACA31270.10026899796095119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)