FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16388630
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG3601.8189894E-129.3309511
GTGTAGG28400.08.87097551
GTCTTAC15400.08.2706171
TACACTG29250.08.18137555
GCACCGT14500.08.1098866
TAACACG9050.08.0398854
TAGGACC15900.08.0082814
GGACTGT28000.07.99954846
GTTCTAG21700.07.9334591
CCTATAC13700.07.8637923
GTGTAGA22150.07.8354711
GTCTAGG22700.07.76894241
TGGACCG9750.07.75077725
TATACTG18800.07.7416165
CTAGGAC22000.07.6952823
TAGACTG20550.07.695255
AGGACCG16750.07.602965
TACACCG8000.07.52202135
TCTACAC21350.07.47082953
CTAACAC27850.07.43529753
Produced by FastQC (version 0.11.5)