FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720024
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC168030.6177519021890984No Hit
CTCCTAGTGAACGTAA141560.5204365843830789No Hit
TAGCTTTACCCTATCC125560.4616135739978765No Hit
CCAGGACTGTAACTCG96810.3559159772119658No Hit
ATCTGTACTGGGACTA91490.3363573262588859No Hit
GTTCTCGACCGTAGAG85660.3149236918497778No Hit
CTTCCTTTGGCTCAGC84670.31128401808219336No Hit
CGATCAAACCCGGCTA78920.29014449872501125No Hit
GGGGGGGGGGGGGGGG68960.2535271747602227No Hit
TGCTGCTACTTTCTTG67600.2485272188774805No Hit
ACAGCCGACTTGCGTA64470.23701996747087528No Hit
CGACCAGACGCGCTGT64020.23536557030379143No Hit
AACTTACCTCACCTAA57830.21260841816101622No Hit
GGCAACAGAATAGCAA56820.2088952156304503No Hit
ATCGAAAGATTTCCGC55420.2037482022217451No Hit
CGCGTTGGAGCGGTTC55000.20220409819913351No Hit
GTAACTGTGTGAAAGT53520.19676296973850232No Hit
CCCTGAACTAGCTTTG52810.19415269865265894No Hit
AGTGGAGTGAGTTGAA51530.18944685782184273No Hit
CAATTCGGATAACGGG50280.1848513101354988No Hit
GGGTAGAACCAGTCTA46670.1715793684173375No Hit
AAGAACCCTTAACCCA45020.16551324547136348No Hit
TTCCTAAACTAATGCC43780.1609544621665103No Hit
GTGAAGCGATCCCAGG43700.1606603471145843No Hit
CGCGATCGATATCTGG43330.15930006499942648No Hit
TTTAGTCTGAGCAATA43020.1581603691732132No Hit
TCCGGGAACGTTCTCC41740.15345452834239698No Hit
TGCAGATACTGCCACG40340.14830751493369176No Hit
TGGAACACTCCTAGTA40300.14816045740772876No Hit
CTTTAACTGTCCGTAA40040.1472045834889692No Hit
CACATCCTGAGCCTAT39830.14643253147766344No Hit
TGATAGAGAGACTGTT39500.14521930688846862No Hit
CATAGGTACAAGGTGC38790.14260903580262527No Hit
AGGTGTGGATCATGTG38070.13996200033529116No Hit
AGTCCTGCTCGAACGA37900.13933700584994838No Hit
GCAACTTGAAGGTGCA37670.1384914250756611No Hit
TCTCAGAGATGCATGT37260.13698408543454027No Hit
TGCAGTATGACACCGC37030.13613850466025298No Hit
CTATTGGGACTTGTGA36580.13448410749316916No Hit
ATTTCTGACGGGTAGT36000.1323517733667056No Hit
CTTAGATCTTGTCACA35410.13018267485875124No Hit
TGGCAATTGAGTGGTG35140.12919003655850095No Hit
TACGTTTACTGCACCG35090.1290062146510472No Hit
AGAGTACCTTAATCGC35010.1287120995991212No Hit
ATGTACGTGTACTTTC34920.1283812201657044No Hit
TCTATGGGATGCTCAA34580.12713123119501887No Hit
TGAACTGTGAACGTAA34010.12503566145004602No Hit
TCAAAGCACTCTGCAA33910.12466801763513852No Hit
TGAGGGATGTACGTTG33760.12411655191277725No Hit
GGTCGCATGGTACTAC33540.1233077355199807No Hit
GGTCTAAACTCCTACC33450.12297685608656395No Hit
CGAGTCTCTGCTACGC33160.12191068902333214No Hit
TTCTTAGACGTCCCGA32850.12077099319711886No Hit
TACTGTTTGCTTAAGC32540.11963129737090555No Hit
GGGTGTGCTCGCAAAG32520.11955776860792405No Hit
GCATGATACTTGCGTA31740.11669014685164543No Hit
CTACAACGAATCTGTG31570.11606515236630266No Hit
TCCATTTGAAATCTGC31410.11547692226245063No Hit
GAGTGTTACACCTGCG31190.1146681058696541No Hit
GGGATGTGACTGGAAG31040.11411664014729281No Hit
TCCGGGTGAACACCTA30470.11202107040231997No Hit
CTAGCTCACGAATAGT30390.11172695535039397No Hit
CTCAATTTGGAATCTC30260.1112490183910142No Hit
CACCAGGTGCGCCACA29990.1102563800907639No Hit
AGACTACTGGCCCTAG29960.11014608694629165No Hit
TTGCGGTCTTTTGGGT29910.1099622650388379No Hit
CATTCTATGCCTAGCG29890.10988873627585638No Hit
GTAATCTCTACACAAA29610.10885933359411534No Hit
TCGGACCGATAGGCGA29330.1078299309123743No Hit
CGAGTCTCTTGGAACG29160.10720493642703154No Hit
CTTTCGTCTCGAACGA28860.10610200498230898No Hit
TGCTAGTGAAGGCGTA28720.10558730364143847No Hit
TGTAAGCACGCGCTGT28580.10507260230056793No Hit
CACATTACTGATGGAC28470.10466819410416967No Hit
GGCTGACTGAAACTGA28470.10466819410416967No Hit
AGCCAATTGTTATGGC28200.10367555580391938No Hit
ACCAAGTTGAAGCTAA28160.10352849827795638No Hit
ATGTCCAGACCGCTAG27830.10231527368876156No Hit
TAGTACCGAACGAGCA27810.10224174492578007No Hit
AGGCGATGACCTGCGA27610.10150645729596504No Hit
ATAGCCAACCGTTCTC27540.10124910662552979No Hit
CATAGTCGAAGATTCC27340.10051381899571475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)