FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4209089
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA410095197.43084548699255No Hit
GCCAAAAA145150.3448489685060116No Hit
CCCAACAA136650.32465457489732336RNA PCR Primer, Index 36 (100% over 8bp)
GCCCACAA89570.2128013924153184No Hit
GACAACAA78780.18716639158734824No Hit
GCCACCAA76160.18094176673384668No Hit
GCAAACAA74000.17581001494622708No Hit
GCCAACCA63450.15074520876132577No Hit
ACCAACAA54660.12986182995892936No Hit
TCCAACAA53690.1275572932765261No Hit
GCCAACAC46920.11147305271995912No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)