FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3748289
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT364832997.33318322039736No Hit
TCACTCCA229080.6111588514119376No Hit
ACACTCCT171680.4580223136476403No Hit
GCACTCCT125650.3352196162035531No Hit
TCACACCT72080.1923010739033196No Hit
TCACTACT54950.14660022212801627No Hit
TCACTCAT52460.13995719113440824RNA PCR Primer, Index 45 (100% over 8bp)
TCCCTCCT43150.11511919171654052No Hit
TCAATCCT39270.104767802055818No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)