FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3748289
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT658221.7560545624950477No Hit
GAAGCAGTGGAACCTT232000.6189490724968112No Hit
GTTCTTCCTAACGTAA191560.5110598462391774No Hit
CATGGAGGATCGACAT157700.42072529626184113No Hit
CGCTTAAGATAATAGG157680.4206719385831776No Hit
TGTCCCATGAGACAAT151990.40549167900340666No Hit
AAAGCATGATCTTTGT144420.38529579762926497No Hit
TGAGCGCACAGGACAC141990.3788128396716475No Hit
TAATTGCCTGTCTGGC130590.3483989628334421No Hit
TGTCGAAACTTCGCAA126940.33866118647735005No Hit
GATACGTACCGCTCGA120890.3225204886816358No Hit
GGAAGGACTAGGTAAT108770.2901857354115438No Hit
CATGTCAGAGGTTTGT101280.2702032847520562No Hit
AATGATGGAAAGCTAA101040.269562992608094No Hit
TCGACCTTGTTTCGCC97300.25958510669801604No Hit
GGGGGGGGGGGGGGGG95520.25483627329696296No Hit
GTGAGATCTCGATTAC81100.21636538698056634No Hit
ATGGGTACTTGGCTGC78560.20958896179029954No Hit
AAACGACCTAGGGAGG76480.20403976320929362No Hit
AGCCTACCTTAACTCG72680.19390180426322517No Hit
TGTTCCTCTTTATGGC71700.19128727800871279No Hit
TTGCGATGACATTCAG71440.19059362818608705No Hit
GCTATGATGACCTGCG70360.18771231353825704No Hit
CAGCTCACTGTTTACG68950.18395059719247903No Hit
CTGCCTTGACCATAAC66940.17858815048679544No Hit
CAATGTGACCAACGCC66500.17741428155619804No Hit
CCTGTTGACCCTAGTA65440.17458632458703158No Hit
TAACCAGACATCACAC65280.17415946315772343No Hit
CATGATGTGTCATGTG63110.1683701550227317No Hit
CACAGCTCTGGACTGA62190.16591570180420986No Hit
ATGCCGCGATGCCACG61840.16498194242759828No Hit
ATGGGTACTTTCGGGC60340.16098011652783442No Hit
CTATTCAACCGAACGA59440.1585790209879761No Hit
TGGGCCATGCGTTCTC59350.15833891143399026No Hit
CATGCAAACGAAGGCT59210.15796540768334566No Hit
TTGTTTGTGATACGCT58580.15628464080544485No Hit
ATTAGCGTGGTCGGTC57560.1535633991936054No Hit
AGAACGCGAGGAATAT57370.153056501246302No Hit
AGCTTGTGACTCACTC56740.15137573436840115No Hit
CATTAGTCTATTCTGG55440.14790748525527248No Hit
CCGCTATTGTTCGCAA54870.14638679141336222No Hit
CACTATACTACGCCAC54500.1453996743580871No Hit
AACCAACACCGGAATG53930.14387898051617684No Hit
GGGCTGACTAATACAG53090.14163795801230908No Hit
ATAGGAAACAGACTTC52630.14041073140304816No Hit
AGCTCGGACCTTAAGC51640.137769526309204No Hit
CCCAATGCTGGAATGC50900.13579529219865386No Hit
TTGACGTGACCTCAAA50520.134781496304047No Hit
AACGTCCCTTACAGAA50490.13470145978605172No Hit
CTGATTACTGCTTTGA49970.13331416014080022No Hit
GGTAGCCTGTGCCACG49890.13310072942614617No Hit
GCAGATACTCGGCTCA49770.13278058335416507No Hit
CTGCCATTGCACAGTT49430.13187350281688526No Hit
ATAGCCAACATGGCCC49130.13107313763693246No Hit
TTGTAACCTCATCCTC48920.13051288201096553No Hit
CCTACGGGATGGAACG48900.130459524332302No Hit
TCAGGCTCTTAAAGCA48570.12957912263435398No Hit
TGGAGTTACAAGTCTC47440.1265644137898652No Hit
CGCTGAGTGTTAGCTG46950.12525715066260898No Hit
ATCTTACTGCGCACTG46870.12504371994795493No Hit
CGCTTACTGGCCACGT46860.12501704110862316No Hit
GGGATCAGATGAGTAA46730.12467021619731028No Hit
TGTTTGTTGCGCTCGA46310.12354970494537641No Hit
CGATCAAACCGATCGC46140.1230961646767365No Hit
GCATCTCTGAGCCTAT46130.12306948583740475No Hit
CGCTTCCCTGCGCTGT45790.12216240530012494No Hit
TCCAAAGTGAAGGCCA45420.12117528824484985No Hit
TTCTAACACTACGTTG45210.1206150326188829No Hit
AGCCGGTACGCGCAAA44880.11973463092093486No Hit
TGGGTGCACTTCTGAG44430.11853408315100569No Hit
CATGTTACTAACGTGG44040.1174936084170671No Hit
CTCGGATCTTTCTGAG43920.11717346234508598No Hit
GATACCATGTCTGGAT43160.11514587055587229No Hit
ACCTGCTTGCCAAACT42860.11434550537591953No Hit
CGAGTCTCTTACTTTC42750.11405203814327017No Hit
TACGCCTTGGGTTGAG42600.11365185555329378No Hit
TACAACCGATTCCATT42390.11309159992732684No Hit
AGTGGTACTCAACGCC42280.11279813269467749No Hit
CCTCCCTTGTTTCGCC41850.11165094260341185No Hit
CTTAGGCCTTATGACC41440.11055711019080973No Hit
CCTTCAGTGTAGCTTG41290.11015692760083333No Hit
AACAGCTACTCGAGAG41200.1099168180468475No Hit
AGAACAATGAGTCGGA41190.10989013920751575No Hit
AACCACTGAAAACATG40940.10922316822422178No Hit
ATTGAGAGATGATCAC40770.10876962795558186No Hit
GTGTACTGAATCACAC40550.10818269349028317No Hit
GCATGGGCTATGTGGG40510.10807597813295614No Hit
ATCGCAATGTAACTCG40450.10791590509696557No Hit
TTCACGCTGTGGGCAA40280.10746236482832568No Hit
GTCCTGACTTTACGAC40220.10730229179233511No Hit
CCTTCTTTGGATCATC40180.10719557643500807No Hit
TGAGATAGACTCATCC40020.10676871500569993No Hit
TTCTATGACCAACGCC39960.10660864196970939No Hit
GTTGCTAGACGCTCGA39880.1063952112550553No Hit
TATCTCCCTGGAATTA39680.10586163446842012No Hit
TCCGGGATGCGAGTTT39210.10460772901982746No Hit
TGGTCCTTGTTTCTGT39080.10426090410851459No Hit
CATGTCAGAACAGGTG39040.10415418875118754No Hit
ACCTCCATGGAATTGA38940.10388740035786996No Hit
AGGTTACTGGTCCCGA38160.10180645088999274No Hit
AGAACATGAAAGCTAA37810.10087269151338117No Hit
TCCCAGACTCTCATTT37800.10084601267404941No Hit
AGAGAATTGCCCGCAA37610.10033911472674599No Hit
CCATGATGAACAGGCA37590.10028575704808247No Hit
GCATATCGAGCCGCTA37550.10017904169075542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)