FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612753
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG250531495.88790061670582No Hit
GGCCAGGG306351.1725180298329003ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA303801.1627582094442146No Hit
GGACAGGC50880.19473712210836616No Hit
GGACACGG43200.16534283952597129No Hit
GGAAAGGG35330.13522135464010568No Hit
GGACAGCG33590.12856171249253184No Hit
CGACAGGG32620.12484915336428665No Hit
GGTCAGGG30460.11658201138798807No Hit
GCACAGGG28590.10942480976961848No Hit
GGGCAGGG27630.10575052444681912No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)