FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5699888
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA557634497.83251881440478No Hit
CCCAACAA188950.33149774171001256RNA PCR Primer, Index 36 (100% over 8bp)
GCCAAAAA155480.27277728965902487No Hit
GACAACAA109220.1916178002094076No Hit
GCCCACAA100320.17600345831356687No Hit
GCAAACAA83950.14728359574784627No Hit
GCCACCAA76940.13498510847932452No Hit
ACCAACAA70630.1239147155172172No Hit
GCCAACAC67500.11842337954710688No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)