FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4401002
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC262850.5972503534422388No Hit
CTCCTAGTGAACGTAA226790.515314467023646No Hit
CGACCAGACGCGCTGT217080.4932513095881347No Hit
TAGCTTTACCCTATCC197400.4485342201616813No Hit
CCAGGACTGTAACTCG155540.3534195167373248No Hit
ATCTGTACTGGGACTA143100.3251532264697903No Hit
CTTCCTTTGGCTCAGC138240.31411028670289176No Hit
GTTCTCGACCGTAGAG137700.31288329339545856No Hit
CGATCAAACCCGGCTA126790.28809348416565134No Hit
AGACCATTGAATCCAC123960.2816631303507701No Hit
TGCTGCTACTTTCTTG115100.2615313512695518No Hit
GGGGGGGGGGGGGGGG106280.24149046058147666No Hit
ACAGCCGACTTGCGTA104600.23767314806946238No Hit
AACTTACCTCACCTAA94650.2150646602750919No Hit
CGCGTTGGAGCGGTTC92090.2092478031139272No Hit
NNNNNNNNNNNNNNNN90130.20479427184991053No Hit
ATCGAAAGATTTCCGC86990.19765953298816952No Hit
GGCAACAGAATAGCAA85120.193410500608725No Hit
CCCTGAACTAGCTTTG83830.19047934992985688No Hit
GTAACTGTGTGAAAGT83490.1897067985881397No Hit
AGTGGAGTGAGTTGAA81730.18570770928983898No Hit
CAATTCGGATAACGGG79460.1805497929789625No Hit
GGGTAGAACCAGTCTA75490.17152911995950015No Hit
AAGAACCCTTAACCCA73120.16614398266576566No Hit
TTCCTAAACTAATGCC72150.1639399391320431No Hit
CGCGATCGATATCTGG70300.1597363509491702No Hit
TTTAGTCTGAGCAATA68460.15555548486458312No Hit
GTGAAGCGATCCCAGG68290.15516920919372454No Hit
TCCGGGAACGTTCTCC67600.15360138441200435No Hit
TGGAACACTCCTAGTA67300.15291972146343039No Hit
TGCAGATACTGCCACG65450.14871613328055747No Hit
TGATAGAGAGACTGTT65310.14839802390455628No Hit
CTTTAACTGTCCGTAA64210.14589859309311834No Hit
CACATCCTGAGCCTAT63140.1434673285765378No Hit
AGTCCTGCTCGAACGA62290.14153595022224486No Hit
AGGTGTGGATCATGTG60620.13774135980851632No Hit
TGCAGTATGACACCGC60500.13746869462908676No Hit
TCTCAGAGATGCATGT60260.13692336427022755No Hit
CTATTGGGACTTGTGA59910.1361280908302246No Hit
CATAGGTACAAGGTGC59530.1352646510953642No Hit
TACGTTTACTGCACCG59420.13501470801422039No Hit
ATTTCTGACGGGTAGT58200.13224261202335286No Hit
CTTAGATCTTGTCACA58180.13219716782678126No Hit
GCAACTTGAAGGTGCA58020.13183361425420848No Hit
TCAAAGCACTCTGCAA57610.1309020082244907No Hit
GAGTGTTACACCTGCG56440.12824352272505216No Hit
CGAGTCTCTGCTACGC56000.12724375040047697No Hit
AGAGTACCTTAATCGC55910.1270392515159048No Hit
TGAACTGTGAACGTAA55770.1267211421399036No Hit
TCTATGGGATGCTCAA55660.1264711990587598No Hit
ATGTACGTGTACTTTC55580.12628942227247342No Hit
TGGCAATTGAGTGGTG55190.12540326043932723No Hit
TTCTTAGACGTCCCGA55100.12519876155475504No Hit
TGAGGGATGTACGTTG53970.12263116444845969No Hit
GGTCTAAACTCCTACC53670.1219495014998857No Hit
GGGTGTGCTCGCAAAG52780.11992723475244957No Hit
TACTGTTTGCTTAAGC51540.11710969456501041No Hit
TCCATTTGAAATCTGC51240.11642803161643644No Hit
GGGATGTGACTGGAAG50800.11542825929186126No Hit
CACCAGGTGCGCCACA50800.11542825929186126No Hit
CTACAACGAATCTGTG50160.11397404500157009No Hit
GGTCGCATGGTACTAC50130.1139058787067127No Hit
CTAGCTCACGAATAGT50130.1139058787067127No Hit
TCCGGGTGAACACCTA50040.11370137982214051No Hit
CTCAATTTGGAATCTC49370.11217899923699193No Hit
AGACTACTGGCCCTAG48860.11102017222441617No Hit
GTAATCTCTACACAAA48830.11095200592955877No Hit
AGCCAATTGTTATGGC48340.1098386231135546No Hit
TTGCGGTCTTTTGGGT48230.10958868003241082No Hit
CTTTCGTCTCGAACGA48040.10915696016498061No Hit
TCGGACCGATAGGCGA48020.10911151596840901No Hit
CATTCTATGCCTAGCG47630.10822535413526284No Hit
CACATTACTGATGGAC47480.10788452266097584No Hit
ACCAAGTTGAAGCTAA46630.1059531443066829No Hit
CGAGTCTCTTGGAACG46560.1057940896186823No Hit
TGCGCAGACATCAGGG46310.10522603716153729No Hit
TGTAAGCACGCGCTGT46260.10511242667010831No Hit
ACCTCAGCTAATCCGT46030.10458981840953492No Hit
ATGTCCAGACCGCTAG45910.10431715323010533No Hit
CTAACAGTGCATGATC45900.10429443113181952No Hit
TGCTAGTGAAGGCGTA45840.10415809854210474No Hit
GGCTGACTGAAACTGA45670.10377182287124614No Hit
CATAGTCGAAGATTCC45630.10368093447810293No Hit
AGGCGATGACCTGCGA45040.10234033067924077No Hit
TAGTACCGAACGAGCA44710.10159050143580939No Hit
TCATCATTGGACTCGC44410.1009088384872354No Hit
ATAGCCAACCGTTCTC44270.10059072911123422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)