FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987541
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT354931.785774482136469No Hit
GAAGCAGTGGAACCTT118430.5958619218421155No Hit
GTTCTTCCTAACGTAA107010.5384039876410096No Hit
CGCTTAAGATAATAGG81580.41045694151718126No Hit
CATGGAGGATCGACAT80950.4072871955848961No Hit
TGTCCCATGAGACAAT76850.3866586903112942No Hit
GATACGTACCGCTCGA75560.3801682581642341No Hit
AAAGCATGATCTTTGT75520.37996700445424775No Hit
TGAGCGCACAGGACAC69500.34967832110130054No Hit
TGTCGAAACTTCGCAA67080.33750247164712577No Hit
TAATTGCCTGTCTGGC66400.33408115857735765No Hit
GGGGGGGGGGGGGGGG62320.31355328015874895No Hit
GGAAGGACTAGGTAAT60230.3030377738119616No Hit
AATGATGGAAAGCTAA54100.27219564275655195No Hit
CATGTCAGAGGTTTGT53650.26993153851920537No Hit
TCGACCTTGTTTCGCC48090.24195727283110136No Hit
GTGAGATCTCGATTAC45010.22646073716215162No Hit
GCTATGATGACCTGCG41890.21076294778321555No Hit
ATGGGTACTTGGCTGC40840.20548003789607358No Hit
CAGCTCACTGTTTACG38900.19571923296173513No Hit
AAACGACCTAGGGAGG37800.19018475593711023No Hit
AGCCTACCTTAACTCG36940.18585780117240347No Hit
TTGCGATGACATTCAG36940.18585780117240347No Hit
TGTTCCTCTTTATGGC36370.18298993580509784No Hit
CTGCCTTGACCATAAC35020.1761976230930582No Hit
CAATGTGACCAACGCC33550.16880154925105947No Hit
CCTGTTGACCCTAGTA33210.16709089271617542No Hit
CATGATGTGTCATGTG33100.16653744501371293No Hit
TAACCAGACATCACAC33020.1661349375937402No Hit
AGCTTGTGACTCACTC32940.1657324301737675No Hit
CACAGCTCTGGACTGA32880.16543054960878795No Hit
CATGCAAACGAAGGCT32260.16231111710399937No Hit
ATGCCGCGATGCCACG32070.16135516198156416No Hit
ATGGGTACTTTCGGGC30850.15521692382698019No Hit
CTATTCAACCGAACGA30820.15506598354449042No Hit
ATTAGCGTGGTCGGTC29650.14917931252738936No Hit
TTGTTTGTGATACGCT29620.1490283722448996No Hit
CATTAGTCTATTCTGG29560.14872649167992005No Hit
CACTATACTACGCCAC29490.14837429768744392No Hit
AGAACGCGAGGAATAT29460.14822335740495415No Hit
GCATCTCTGAGCCTAT28820.14500329804517242No Hit
GGGTCGTACCGCTCGA27350.13760722420317367No Hit
AGCTCGGACCTTAAGC27240.13705377650071118No Hit
GCAGATACTCGGCTCA27030.1359971945232828No Hit
CCGCTATTGTTCGCAA26860.13514186625584074No Hit
CCCAATGCTGGAATGC26850.13509155282834417No Hit
TTGTAACCTCATCCTC26690.13428653798839874No Hit
TGGGCCATGCGTTCTC26430.1329783888734874No Hit
AACCAACACCGGAATG26420.13292807544599078No Hit
AACGTCCCTTACAGAA26030.1309658517736238No Hit
ATAGGAAACAGACTTC25940.1305130309261545No Hit
GGGATCAGATGAGTAA25690.12925519523873974No Hit
CTGCCATTGCACAGTT25470.12814829983381473No Hit
TTGACGTGACCTCAAA25350.12754453870385565No Hit
CGATCAAACCGATCGC25290.1272426581388761No Hit
CTGATTACTGCTTTGA25180.12668921043641365No Hit
TGGAGTTACAAGTCTC25010.12583388216897162No Hit
GATACCATGTCTGGAT24920.12538106132150229No Hit
CCTACGGGATGGAACG24850.12502886732902616No Hit
CGCTTACTGGCCACGT24290.12221131538921713No Hit
CGCTGAGTGTTAGCTG24210.1218088079692444No Hit
ATAGCCAACATGGCCC23970.12060128570932625No Hit
GTAAGAAACGACAAAT23920.1203497185718433No Hit
TGTTTGTTGCGCTCGA23790.11969564401438763No Hit
CATGTTACTAACGTGG23640.11894094260193877No Hit
GCGTCGATGCTTGAAC23630.11889062917444218No Hit
TCAGGCTCTTAAAGCA23500.11823655461698651No Hit
TCCAAAGTGAAGGCCA23350.11748185320453768No Hit
ATCTTACTGCGCACTG23210.1167774652195854No Hit
CGCTTCCCTGCGCTGT23040.11592213695214339No Hit
CTCGGATCTTTCTGAG22750.11446304755474226No Hit
TTCTAACACTACGTTG22580.11360771928730024No Hit
GGTAGCCTGTGCCACG22430.11285301787485139No Hit
TGGGTGCACTTCTGAG22310.1122492567448923No Hit
TACAACCGATTCCATT22250.11194737617991277No Hit
GGGCTGACTAATACAG22130.11134361504995369No Hit
CGAGTCTCTTACTTTC22070.11104173448497415No Hit
CTTAGGCCTTATGACC22050.11094110762998097No Hit
AGTGGTACTCAACGCC21870.11003546593504235No Hit
CCTTCAGTGTAGCTTG21830.10983421222505599No Hit
GCGACAAGAAAGGCTG21800.10968327194256622No Hit
TGAGATAGACTCATCC21790.10963295851506961No Hit
CCTCCCTTGTTTCGCC21730.10933107795009007No Hit
ATCGCAATGTAACTCG21710.10923045109509691No Hit
TACGCCTTGGGTTGAG21700.10918013766760032No Hit
GCATAGTACGCTCCTT21690.10912982424010373No Hit
ATTGAGAGATGATCAC21590.10862668996513783No Hit
GCATATCGAGCCGCTA21150.10641289915528786No Hit
AACCACTGAAAACATG21110.1062116454453015No Hit
AACAGCTACTCGAGAG21050.10590976488032197No Hit
CATGTCAGAACAGGTG21020.1057588245978322No Hit
GAAAGTAACCCTGCGA21010.10570851117033561No Hit
GTGTACTGAATCACAC20950.10540663060535607No Hit
AGGTTACTGGTCCCGA20780.10455130233791403No Hit
TATCTCCCTGGAATTA20650.10389722778045836No Hit
TGGTCCTTGTTTCTGT20570.10349472036048565No Hit
TTCACGCTGTGGGCAA20550.10339409350549247No Hit
AGCCGGTACGCGCAAA20450.10289095923052657No Hit
AGAACATGAAAGCTAA20440.10284064580302998No Hit
GTTGCTAGACGCTCGA20400.10263939209304361No Hit
AGAACAATGAGTCGGA20190.10158281011561524No Hit
GGTCAGAGAACCAGTT20090.10107967584064932No Hit
AGAGAATTGCCCGCAA20090.10107967584064932No Hit
TTCTATGACCAACGCC20080.10102936241315273No Hit
CGGTTCACTGGAATGC20020.10072748184817319No Hit
ACCTCCATGGAATTGA19910.1001740341457107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)